Biotinylated Human DPPIV/CD26 Protein, Ultra Low Endotoxin (DPV-HM426B

Express System: HEK293
Product Tag: N-His-Avi
Purity: > 90% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human DPPIV, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-DPPIV Antibody, hFc Tag with the EC50 of 7.6ng/ml determined by ELISA. See testing image for detail.Measured by its ability to cleave the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin (GP-AMC). The specific activity is >8500 pmol/min/µg.
Amino Acid Range: Asn29-Pro766

Catalog #DPV-HM426B-UL

Price

$635⁸⁰

Size

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Biotinylated Human DPPIV/CD26 Protein, Ultra Low Endotoxin (DPV-HM426B-UL) Summary

Product Description	Recombinant Biotinylated Human DPPIV/CD26 Protein is expressed from HEK293 with His tag and Avi tag at the N-terminus.It contains Asn29-Pro766.
Product Background	CD26/dipeptidyl peptidase (DPP)IV is a membrane-bound protein found in many cell types of the body, and a soluble form is present in body fluids. There is longstanding evidence that various primary tumors and also metastases express DPPIV/CD26 to a variable extent.

Protein	DPPIV/CD26
Synonyms	ADCP2; CD26; DPPIV; DPP4; ADABP; TP103; EC 3.4.14.5;EC3.4.14.5
Accession	P27487
Species	Human
Biotinylated	yes
Amino Acid Range	Asn29-Pro766
Molecular Weight	The protein has a predicted MW of 88.27 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human DPPIV, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-DPPIV Antibody, hFc Tag with the EC50 of 7.6ng/ml determined by ELISA. See testing image for detail.Measured by its ability to cleave the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin (GP-AMC). The specific activity is >8500 pmol/min/µg.
Product Tag	N-His-Avi
Fluorophore	Biotin-labeled
Expression System	HEK293
Purity	> 90% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.01 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Human DPPIV, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-DPPIV Antibody, hFc Tag with the EC50 of 7.6ng/ml determined by ELISA.
	Biotinylated Human DPPIV on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
	The purity of Biotinylated Human DPPIV is greater than 95% as determined by SEC-HPLC.