Biotinylated Human E-Selectin/CD62E Protein (ESE-HM401B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Anti-E-Selectin Antibody at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human E-selectin, His Tag with the EC50 of 78.4ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Trp22-Pro556

Catalog: ESE-HM401B

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Product Description Recombinant Biotinylated Human E-Selectin/CD62E Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Trp22-Pro556.
Product Category Recombinant Protein
Target E-Selectin/CD62E
Synonyms CD62E; ELAM; ELAM1; ESEL; E-Selectin; LECAM2; SELE; selectin E; RP1-117P20.2
Gene/Protein ID P16581
Species Human
Exact Sequence Trp22-Pro556
Molecular Weight The protein has a predicted MW of 61.6 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result.
Activity Immobilized Anti-E-Selectin Antibody at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human E-selectin, His Tag with the EC50 of 78.4ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-E-Selectin Antibody at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human E-selectin, His Tag with the EC50 of 78.4ng/ml determined by ELISA.
The purity of Biotinylated Human E-selectin is greater than 95% as determined by SEC-HPLC.
Biotinylated Human E-selectin on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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