Name: Biotinylated Human E-Selectin/CD62E Protein (ESE-HM401B)
Brand: KactusBio
SKU: ESE-HM401B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human E-Selectin/CD62E Protein (ESE-HM401B)

Immobilized Biotinylated Human E-selectin, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-E-Selectin Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA.

Biotinylated Human E-selectin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human E-selectin is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human E-selectin, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-E-Selectin Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Trp22-Pro556

Catalog #ESE-HM401B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human E-Selectin/CD62E Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Trp22-Pro556.
Product Background	E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107 - 115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha.E-selectin mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.
Product Category	Recombinant Protein / Antibody discovery

Protein	E-Selectin/CD62E
Synonyms	CD62E; ELAM; ELAM1; ESEL; E-Selectin; LECAM2; SELE; selectin E; RP1-117P20.2
Accession	P16581
Species	Human
Biotinylated	yes
Amino Acid Range	Trp22-Pro556
Molecular Weight	The protein has a predicted MW of 61.6 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human E-selectin, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-E-Selectin Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Human E-selectin, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-E-Selectin Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA.
	Biotinylated Human E-selectin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human E-selectin is greater than 95% as determined by SEC-HPLC.