Biotinylated Human EMMPRIN/CD147 Protein (EPN-HM447B)

Immobilized Biotinylated Human EMMPRIN, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-EMMPRIN Antibody, hFc Tag with the EC50 of 1.8ng/ml determined by ELISA.

Biotinylated Human EMMPRIN on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human EMMPRIN is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human EMMPRIN, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-EMMPRIN Antibody, hFc Tag with the EC50 of 1.8ng/ml determined by ELISA.
Amino Acid Range: Ala22-His205

Catalog #EPN-HM447B

Price

$578⁰⁰

Size

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Biotinylated Human EMMPRIN/CD147 Protein (EPN-HM447B) Summary

Product Description

Recombinant Biotinylated Human EMMPRIN/CD147 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Ala22-His205.

Product Background

CD147, also known as extracellular matrix metalloproteinase inducer (EMMPRIN) or basigin, is expressed in a variety of cell types. It is involved in the regulation of extracellular matrix (ECM) remodeling during physiological and pathological processes including wound healing, inflammatory diseases, and cancer. CD147 is a diagnostic and therapeutic target in cancer and inflammatory diseases, either directly or indirectly, by targeting CD147 partners.

Biotinylated Human EMMPRIN/CD147 Protein (EPN-HM447B) Specifications/Details

Protein	EMMPRIN/CD147
Synonyms	Basigin; BSG; CD147; 5F7; M6; OK; TCSF
Accession	NP_940991.1
Species	Human
Biotinylated	yes
Amino Acid Range	Ala22-His205
Molecular Weight	The protein has a predicted MW of 23.02 kDa. Due to glycosylation, the protein migrates to 31-45 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human EMMPRIN, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-EMMPRIN Antibody, hFc Tag with the EC50 of 1.8ng/ml determined by ELISA.
Product Tag	C-His-Avi
Fluorophore	Biotin-labeled
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Biotinylated Human EMMPRIN, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-EMMPRIN Antibody, hFc Tag with the EC50 of 1.8ng/ml determined by ELISA.
	Biotinylated Human EMMPRIN on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human EMMPRIN is greater than 95% as determined by SEC-HPLC.