Name: Biotinylated Human Endoglin/CD105 Protein (EDG-HM401B)
Brand: KactusBio
SKU: EDG-HM401B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human Endoglin/CD105 Protein (EDG-HM401B)

Immobilized Biotinylated Human Endoglin, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Endoglin Antibody, hFc Tag with the EC50 of 6.8ng/ml determined by ELISA.

Biotinylated Human Endoglin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human Endoglin is greater than 90% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: Immobilized Biotinylated Human Endoglin, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Endoglin Antibody, hFc Tag with the EC50 of 6.8ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Glu26-Gly586

Catalog #EDG-HM401B

Size

100UG

500UG

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Biotinylated Human Endoglin/CD105 Protein (EDG-HM401B) Summary

Product Description	Recombinant Biotinylated Human Endoglin/CD105 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Glu26-Gly586.
Product Background	Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida (ZP) family of proteins. Endoglin is a vascular endothelium glycoprotein that plays an important role in the regulation of angiogenesis. Required for normal structure and integrity of adult vasculature (PubMed:7894484). Regulates the migration of vascular endothelial cells. Required for normal extraembryonic angiogenesis and for embryonic heart development. May regulate endothelial cell shape changes in response to blood flow, which drive vascular remodeling and establishment of normal vascular morphology during angiogenesis.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Biotinylated Human Endoglin/CD105 Protein (EDG-HM401B) Specifications/Details

Protein	Endoglin/CD105
Synonyms	CD105; Endoglin; ENG; ORW; ORW1; END; HHT1
Accession	P17813-1
Species	Human
Biotinylated	yes
Amino Acid Range	Glu26-Gly586
Molecular Weight	The protein has a predicted MW of 63.7 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human Endoglin, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Endoglin Antibody, hFc Tag with the EC50 of 6.8ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Human Endoglin, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Endoglin Antibody, hFc Tag with the EC50 of 6.8ng/ml determined by ELISA.
	Biotinylated Human Endoglin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human Endoglin is greater than 90% as determined by SEC-HPLC.