Biotinylated Human ENPP-1 Protein (ENP-HM402B)
Biotinylated Human ENPP-1 Protein (ENP-HM402B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Biotinylated Human ENPP-1, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-ENPP-1 Antibody, hFc Tag with the EC50 of 4.5ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Lys98-Asp925
Catalog #ENP-HM402B
Regular price $577.50
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Product Description

Recombinant Biotinylated Human ENPP-1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Lys98-Asp925.

Product Background

Ectonucleotide pyrophosphatase/phosphodiesterase (ENPP)-1 is a membrane-bound protein that catalyzes the hydrolysis of extracellular nucleoside triphosphates to monophosphate and extracellular inorganic pyrophosphate (ePPi). Mechanical stimulation regulates ENPP-1 expression.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Protein

ENPP-1

Synonyms

E-NPP 1; NPPase; ENPP1; NPPS; PC1; PDNP1; Ly-41 Antigen; M6S1; M6S1NPP1; NPP1; PCA1; PCA1ARHR2

Accession

Species

Human

Biotinylated

yes

Amino Acid Range

Lys98-Asp925

Molecular Weight

The protein has a predicted MW of 98.29 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Biotinylated Human ENPP-1, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-ENPP-1 Antibody, hFc Tag with the EC50 of 4.5ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in 20mM Tris,150mM NaCl (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human ENPP-1, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-ENPP-1 Antibody, hFc Tag with the EC50 of 4.5ng/ml determined by ELISA. Immobilized Biotinylated Human ENPP-1, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-ENPP-1 Antibody, hFc Tag with the EC50 of 4.5ng/ml determined by ELISA.
Biotinylated Human ENPP-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human ENPP-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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support@kactusbio.us

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