Name: Biotinylated Human ENPP-1 Protein (ENP-HM402B)
Brand: KactusBio
SKU: ENP-HM402B-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Human ENPP-1 Protein (ENP-HM402B)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Biotinylated Human ENPP-1, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-ENPP-1 Antibody, hFc Tag with the EC50 of 4.5ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Lys98-Asp925

Catalog #ENP-HM402B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human ENPP-1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Lys98-Asp925.
Product Background	Ectonucleotide pyrophosphatase/phosphodiesterase (ENPP)-1 is a membrane-bound protein that catalyzes the hydrolysis of extracellular nucleoside triphosphates to monophosphate and extracellular inorganic pyrophosphate (ePPi). Mechanical stimulation regulates ENPP-1 expression.
Product Category	Recombinant Protein

Target	Biotinylated ENPP-1
Synonyms	E-NPP 1; NPPase; ENPP1; NPPS; PC1; PDNP1; Ly-41 Antigen; M6S1; M6S1NPP1; NPP1; PCA1; PCA1ARHR2
Accession	P22413
Species	Human
Biotinylated	yes
Amino Acid Range	Lys98-Asp925
Molecular Weight	The protein has a predicted MW of 98.29 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human ENPP-1, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-ENPP-1 Antibody, hFc Tag with the EC50 of 4.5ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 20mM Tris,150mM NaCl (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Biotinylated Human ENPP-1, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-ENPP-1 Antibody, hFc Tag with the EC50 of 4.5ng/ml determined by ELISA.
	Biotinylated Human ENPP-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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