Name: Biotinylated Human ENPP-1 Protein (ENP-HM402B)
Brand: KactusBio
SKU: ENP-HM402B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human ENPP-1 Protein (ENP-HM402B)

Immobilized Biotinylated Human ENPP-1, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-ENPP-1 Antibody, hFc Tag with the EC50 of 4.5ng/ml determined by ELISA.

Biotinylated Human ENPP-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Biotinylated Human ENPP-1, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-ENPP-1 Antibody, hFc Tag with the EC50 of 4.5ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Lys98-Asp925

Catalog #ENP-HM402B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human ENPP-1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Lys98-Asp925.
Product Background	Ectonucleotide pyrophosphatase/phosphodiesterase (ENPP)-1 is a membrane-bound protein that catalyzes the hydrolysis of extracellular nucleoside triphosphates to monophosphate and extracellular inorganic pyrophosphate (ePPi). Mechanical stimulation regulates ENPP-1 expression.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	ENPP-1
Synonyms	E-NPP 1; NPPase; ENPP1; NPPS; PC1; PDNP1; Ly-41 Antigen; M6S1; M6S1NPP1; NPP1; PCA1; PCA1ARHR2
Accession	P22413
Species	Human
Biotinylated	yes
Amino Acid Range	Lys98-Asp925
Molecular Weight	The protein has a predicted MW of 98.29 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human ENPP-1, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-ENPP-1 Antibody, hFc Tag with the EC50 of 4.5ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 20mM Tris,150mM NaCl (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Human ENPP-1, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-ENPP-1 Antibody, hFc Tag with the EC50 of 4.5ng/ml determined by ELISA.
	Biotinylated Human ENPP-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.