Regular price
$578.00
Order by 4pm ET for delivery on:
Biotinylated Human ENPP-3 (558-875) Protein (ENP-HM406B) Summary
Product Description |
Recombinant Biotinylated Human ENPP-3 (558-875) Protein is expressed from HEK293 with His tag and Avi tag at the N-terminus.It contains Lys558-Ile875. |
Product Background |
Ectonucleotide pyrophosphatase-phosphodiesterase 3 (ENPP3), a protein detected in the human uterus, has been found to play an important role in the development and invasion of tumours. It was recently discovered that ENPP3 was upregulated during the window of implantation in the human endometrium but its functional relevance remains elusive. |
Product Category |
Recombinant Protein / Antibody discovery / ADC targets |
Biotinylated Human ENPP-3 (558-875) Protein (ENP-HM406B) Specifications/Details
Protein |
ENPP-3 |
Synonyms |
E-NPP 3; NPP3; PD-Ibeta; NPPase; ENPP3; PDNP3; CD203c; B10; gp130RB13-6; PD-Iβ |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Lys558-Ile875 |
Molecular Weight |
The protein has a predicted MW of 39.14 kDa. Due to glycosylation, the protein migrates to 52-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human ENPP-3 (558-875), His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-ENPP3 Antibody, hFc Tag with the EC50 of 91.7ng/ml determined by ELISA. |
Product Tag |
N-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Immobilized Biotinylated Human ENPP-3 (558-875), His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-ENPP3 Antibody, hFc Tag with the EC50 of 91.7ng/ml determined by ELISA. |
|
|
Biotinylated Human ENPP-3 (558-875) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Biotinylated Human ENPP-3 (558-875) is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
Recommended Products
Loading...
Recently viewed
