Product Description |
Recombinant Biotinylated Human EPO R/Erythropoietin R Protein is expressed from HEK293 with hFc tag and Avi tag at the C-terminus.It contains Ala25-Pro250. |
Product Background |
The erythropoietin (epo) receptor is a member of the cytokine receptor family. It is expressed almost exclusively on erythroid precursor cells and controls the development of red blood cells. The epo receptor has no intrinsic kinase activity, but binds intracellular tyrosine kinases to elicit its signals. Alterations in the transmission of the signalling cascade lead to clinically abnormal red blood cell production. |
Product Category |
Recombinant Protein |
Target |
EPO R/Erythropoietin R |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Ala25-Pro250 |
Molecular Weight |
The protein has a predicted MW of 52.58 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human EPO, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human EPO R, hFc Tag with the EC50 of 0.70ug/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-hFc-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human EPO, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human EPO R, hFc Tag with the EC50 of 0.70ug/ml determined by ELISA. |
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Biotinylated Human EPO R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human EPO R is greater than 95% as determined by SEC-HPLC. |
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