Biotinylated Human Fc gamma RI/CD64 Protein (FRI-HM464B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 0.91 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gln16-Pro288
Catalog #FRI-HM464B
Regular price $525.00
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Product Description
Recombinant Biotinylated Human Fc gamma RI/CD64 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln16-Pro288.
Product Background
The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma- RI (also known as CD64), Fc gamma- RII (CD32), and Fc gamma- RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type.
Product Category
Recombinant Protein
Target
Fc gamma RI/CD64
Synonyms
CD64; CD64a; Fc gamma RI; FCG1; Fc-gamma RIA; FCGR1; FcgRI; FCRI; FcRIA; FLJ18345; FCGR1A; IGFR1; Fcr; Fcgr; FCE1A; FcERI
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Gln16-Pro288
Molecular Weight
The protein has a predicted MW of 33.5 kDa. Due to glycosylation, the protein migrates to 55-68 kDa based on Bis-Tris PAGE result.
Activity
The affinity constant of 0.91 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Biotinylated Human Fc gamma RI, His-Avi Tag captured on CM5 Chip via Anti-His Antibody can bind Trastuzumab with an affinity constant of 0.91 nM as determined in SPR assay (Biacore T200).
Biotinylated Human Fc gamma RI on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human Fc gamma RI is greater than 95% as determined by SEC-HPLC.
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