Biotinylated Human Fc gamma RI/CD64 Protein (FRI-HM464B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
  • Activity: The affinity constant of 0.91 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gln16-Pro288
Catalog #FRI-HM464B
Regular price $525.00
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Product Description
Recombinant Biotinylated Human Fc gamma RI/CD64 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln16-Pro288.
Product Background
The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma- RI (also known as CD64), Fc gamma- RII (CD32), and Fc gamma- RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type.
Product Category
Recombinant Protein
Target
Fc gamma RI/CD64
Synonyms
CD64; CD64a; Fc gamma RI; FCG1; Fc-gamma RIA; FCGR1; FcgRI; FCRI; FcRIA; FLJ18345; FCGR1A; IGFR1; Fcr; Fcgr; FCE1A; FcERI; FcγRI; Fc γRI; Fcγ RI; Fc γ RI
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Gln16-Pro288
Molecular Weight
The protein has a predicted MW of 33.5 kDa. Due to glycosylation, the protein migrates to 55-68 kDa based on Bis-Tris PAGE result.
Activity
The affinity constant of 0.91 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Biotinylated Human Fc gamma RI, His-Avi Tag captured on CM5 Chip via Anti-His Antibody can bind Trastuzumab with an affinity constant of 0.91 nM as determined in SPR assay (Biacore T200).
Biotinylated Human Fc gamma RI on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human Fc gamma RI is greater than 95% as determined by SEC-HPLC.

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