Name: Biotinylated Human Fc gamma RI/CD64 Protein (FRI-HM464B)
Brand: KactusBio
SKU: FRI-HM464B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human Fc gamma RI/CD64 Protein (FRI-HM464B)

Biotinylated Human Fc gamma RI, His-Avi Tag captured on CM5 Chip via Anti-His Antibody can bind Trastuzumab with an affinity constant of 0.91 nM as determined in SPR assay (Biacore T200).

Biotinylated Human Fc gamma RI on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human Fc gamma RI is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 0.91 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Gln16-Pro288

Catalog #FRI-HM464B

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Human Fc gamma RI/CD64 Protein (FRI-HM464B) Summary

Product Description	Recombinant Biotinylated Human Fc gamma RI/CD64 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln16-Pro288.
Product Background	The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma- RI (also known as CD64), Fc gamma- RII (CD32), and Fc gamma- RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type.
Product Category	Recombinant Protein / Antibody discovery / Fc receptors

Biotinylated Human Fc gamma RI/CD64 Protein (FRI-HM464B) Specifications/Details

Protein	Fc gamma RI/CD64
Synonyms	CD64; CD64a; Fc gamma RI; FCG1; Fc-gamma RIA; FCGR1; FcgRI; FCRI; FcRIA; FLJ18345; FCGR1A; IGFR1; Fcr; Fcgr; FCE1A; FcERI; FcγRI; Fc γRI; Fcγ RI; Fc γ RI
Accession	P12314-1
Species	Human
Biotinylated	yes
Amino Acid Range	Gln16-Pro288
Molecular Weight	The protein has a predicted MW of 33.5 kDa. Due to glycosylation, the protein migrates to 55-68 kDa based on Bis-Tris PAGE result.
Activity	The affinity constant of 0.91 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Biotinylated Human Fc gamma RI, His-Avi Tag captured on CM5 Chip via Anti-His Antibody can bind Trastuzumab with an affinity constant of 0.91 nM as determined in SPR assay (Biacore T200).
	Biotinylated Human Fc gamma RI on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human Fc gamma RI is greater than 95% as determined by SEC-HPLC.