Biotinylated Human Fc gamma RIIA/CD32a (H167) Protein (CDA-HM432B)
Catalog: CDA-HM432B
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| Product Description | Recombinant Biotinylated Human Fc gamma RIIA/CD32a (H167) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala36-Ile218(H167). |
| Product Category | Recombinant Protein |
| Target | Fc gamma RIIA/CD32a (H167) |
| Synonyms | CD32MGC23887; CDw32fc-gamma-RIIa; Fc gamma RIIA; FCG2; Fc-gamma-RIIa; FcGR; FCGR2; FCGR2A; FCGR2A1; FcgRIIA; FCRIIA; fcRII-a; IGFR2MGC30032; IgG Fc receptor II-a; FCG2; CD32A |
| Gene/Protein ID | P12318-1 |
| Species | Human |
| Exact Sequence | Ala36-Ile218(H167) |
| Molecular Weight | The protein has a predicted MW of 23.2 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result. |
| Activity | The affinity constant of 1.44 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Biotinylated Human Fc gamma RIIA (H167) , His-Avi Tag captured on CM5 Chip via Anti-his antibody can bind Rituximab with an affinity constant of 1.44 uM as determined in SPR assay (Biacore T200) (QC Test). |
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Rituximab captured on CM5 Chip via Protein A can bind Biotinylated Human Fc gamma RIIA (H167) , His-Avi Tag with an affinity constant of 0.680 uM as determined in SPR assay (Biacore T200). |
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The purity of Human Fc gamma RIIA (H167) is greater than 95% as determined by SEC-HPLC. |
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Human Fc gamma RIIA (H167) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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