Product Description |
Recombinant Biotinylated Human Fc gamma RIIA/CD32a (H167) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala36-Ile218(H167). |
Product Background |
The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10e-8-10e-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10e-6-10e-7 M) only recognizing IgG aggregates surrounding multivalent antigens. |
Product Category |
Recombinant Protein / Antibody discovery / Fc receptors |
Protein |
Fc gamma RIIA/CD32a (H167) |
Synonyms |
Fc gamma RIIA; FCG2; FCGR2; FCGR2A; FCGR2A1; FcgRIIA; FCRIIA; fcRII-a; FCG2; CD32A; FcγRIIA; Fc γRIIA; Fcγ RIIA; Fc γ RIIA |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Ala36-Ile218(H167) |
Molecular Weight |
The protein has a predicted MW of 23.2 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 1.44 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His-Avi |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Biotinylated Human Fc gamma RIIA (H167) , His-Avi Tag captured on CM5 Chip via Anti-his antibody can bind Rituximab with an affinity constant of 1.44 uM as determined in SPR assay (Biacore T200) (QC Test). |
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Rituximab captured on CM5 Chip via Protein A can bind Biotinylated Human Fc gamma RIIA (H167) , His-Avi Tag with an affinity constant of 0.680 uM as determined in SPR assay (Biacore T200). |
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Human Fc gamma RIIA (H167) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human Fc gamma RIIA (H167) is greater than 95% as determined by SEC-HPLC. |
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