Biotinylated Human Fc gamma RIIB/CD32b Protein (CDB-HM401B)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: The affinity constant of 3.97 μM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Ala46-Pro217

Catalog: CDB-HM401B

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Product Description Recombinant Biotinylated Human Fc gamma RIIB/CD32b Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.; It contains Ala46-Pro217.
Product Category Recombinant Protein
Target Fc gamma RIIB /CD32b
Synonyms Fc gamma Receptor IIB/C; CD32; CD32b; CDw32; FCGR2B/C; CD32b/c; FcRII-b/c; FCG2; IGFR2; CD32A; Fc gamma RII; Fc gamma RIIB; FCG2 ; FcGR; FCGR2; FCGR21; FCGR2A
Gene/Protein ID P31994-1
Species Human
Exact Sequence Ala46-Pro217
Molecular Weight The protein has a predicted MW of 22.5 kDa. Due to glycosylation, the protein migrates to 32-37 kDa based on Tris-Bis PAGE result.
Activity The affinity constant of 3.97 μM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Rituximab captured on CM5 Chip via Protein A can bind Biotinylated Human Fc gamma RIIB, His-Avi Tag with an affinity constant of 3.97 μM as determined in SPR assay (Biacore T200).
The purity of Biotinylated Human Fc gamma RIIB/CD32b is greater than 95% as determined by SEC-HPLC.
Biotinylated Human Fc gamma RIIB/CD32b on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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