Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein (CDA-HM416B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Anti-Fc gamma RIIIA Antibody, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human Fc gamma RIIIA (F176) , His Tag with the EC50 of 37.7ng/ml determined by ELISA (QC Test). The affinity constant of 0.14uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Gly17-Gln208(F176)

Catalog: CDA-HM416B

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Product Description Recombinant Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus..It contains Gly17-Gln208(F176).
Product Category Recombinant Protein
Target Fc gamma RIIIA/CD16a (F176)
Synonyms CD16FCRIIIA; Fc fragment of IgG; Fc gamma RIIIA; Fc-gamma RIII-alpha; FCGR3; FCGR3A; FCGRIII; FcgRIIIA; FcR-10; CD16A; FCG3; IGFR3; FcRIIIa; FcRIII; CD16; IMD20
Gene/Protein ID P08637-1
Species Human
Exact Sequence Gly17-Gln208(F176)
Molecular Weight The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 48-58 kDa based on Tris-Bis PAGE result.
Activity Immobilized Anti-Fc gamma RIIIA Antibody, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human Fc gamma RIIIA (F176) , His Tag with the EC50 of 37.7ng/ml determined by ELISA (QC Test). The affinity constant of 0.14uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His-Avi
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Biotinylated Human Fc gamma RIIIA (F176) , His Tag captured on CM5 Chip via Anti-His Antibody can bind Rituximab, hFc Tag with an affinity constant of 0.14uM as determined in SPR assay (Biacore T200).
Immobilized Anti-Fc gamma RIIIA Antibody, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human Fc gamma RIIIA (F176) , His Tag with the EC50 of 37.7ng/ml determined by ELISA (QC Test).
The purity of Biotinylated Human Fc gamma RIIIA (F176) is greater than 95% as determined by SEC-HPLC.
Biotinylated Human Fc gamma RIIIA (F176) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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