Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein (CDA-HM416B)
Catalog: CDA-HM416B
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| Product Description | Recombinant Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus..It contains Gly17-Gln208(F176). |
| Product Category | Recombinant Protein |
| Target | Fc gamma RIIIA/CD16a (F176) |
| Synonyms | CD16FCRIIIA; Fc fragment of IgG; Fc gamma RIIIA; Fc-gamma RIII-alpha; FCGR3; FCGR3A; FCGRIII; FcgRIIIA; FcR-10; CD16A; FCG3; IGFR3; FcRIIIa; FcRIII; CD16; IMD20 |
| Gene/Protein ID | P08637-1 |
| Species | Human |
| Exact Sequence | Gly17-Gln208(F176) |
| Molecular Weight | The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 48-58 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Anti-Fc gamma RIIIA Antibody, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human Fc gamma RIIIA (F176) , His Tag with the EC50 of 37.7ng/ml determined by ELISA (QC Test). The affinity constant of 0.14uM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Biotinylated Human Fc gamma RIIIA (F176) , His Tag captured on CM5 Chip via Anti-His Antibody can bind Rituximab, hFc Tag with an affinity constant of 0.14uM as determined in SPR assay (Biacore T200). |
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Immobilized Anti-Fc gamma RIIIA Antibody, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human Fc gamma RIIIA (F176) , His Tag with the EC50 of 37.7ng/ml determined by ELISA (QC Test). |
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The purity of Biotinylated Human Fc gamma RIIIA (F176) is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Human Fc gamma RIIIA (F176) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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