Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein (CDA-HM416B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Anti-Fc gamma RIIIA Antibody, hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human Fc gamma RIIIA (F176), His-Avi Tag with the EC50 of 18.9ng/ml determined by ELISA (QC Test). The affinity constant of 0.14uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gly17-Gln208(F176)
Catalog #CDA-HM416B
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Product Description
 Recombinant Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus..It contains Gly17-Gln208(F176).
Product Background
 Human Fc gamma RIIIA/CD16a Protein is a receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.
Product Category
 Recombinant Protein
Target
 Fc gamma RIIIA/CD16a (F176)
Synonyms
 Fc gamma RIIIA; FCGR3; FCGR3A; FCGRIII; FcgRIIIA; FcR-10; CD16A; FCG3; IGFR3; FcRIIIa; FcRIII; CD16; IMD20
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Gly17-Gln208(F176)
Molecular Weight
 The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 48-58 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Anti-Fc gamma RIIIA Antibody, hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human Fc gamma RIIIA (F176), His-Avi Tag with the EC50 of 18.9ng/ml determined by ELISA (QC Test). The affinity constant of 0.14uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His-Avi
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Biotinylated Human Fc gamma RIIIA (F176), His Tag captured on CM5 Chip via Anti-His Antibody can bind Rituximab, hFc Tag with an affinity constant of 0.14uM as determined in SPR assay (Biacore T200).
Immobilized Anti-Fc gamma RIIIA Antibody, hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human Fc gamma RIIIA (F176), His-Avi Tag with the EC50 of 18.9ng/ml determined by ELISA (QC Test).
Biotinylated Human Fc gamma RIIIA (F176) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human Fc gamma RIIIA (F176) is greater than 95% as determined by SEC-HPLC.

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