Name: Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein (CDA-HM416B)
Brand: KactusBio
SKU: CDA-HM416B-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein (CDA-HM416B)

Biotinylated Human Fc gamma RIIIA (F176), His Tag captured on CM5 Chip via Anti-His Antibody can bind Rituximab, hFc Tag with an affinity constant of 0.14uM as determined in SPR assay (Biacore T200).

Immobilized Anti-Fc gamma RIIIA Antibody, hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human Fc gamma RIIIA (F176), His-Avi Tag with the EC50 of 18.9ng/ml determined by ELISA (QC Test).

Biotinylated Human Fc gamma RIIIA (F176) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human Fc gamma RIIIA (F176) is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Anti-Fc gamma RIIIA Antibody, hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human Fc gamma RIIIA (F176), His-Avi Tag with the EC50 of 18.9ng/ml determined by ELISA (QC Test). The affinity constant of 0.14uM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Gly17-Gln208(F176)

Catalog #CDA-HM416B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus..It contains Gly17-Gln208(F176).
Product Background	Human Fc gamma RIIIA/CD16a Protein is a receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.
Product Category	Recombinant Protein / Antibody discovery / Fc receptors

Protein	Fc gamma RIIIA/CD16a (F176)
Synonyms	Fc gamma RIIIA; FCGR3; FCGR3A; FCGRIII; FcgRIIIA; FcR-10; CD16A; FCG3; IGFR3; FcRIIIa; FcRIII; CD16; IMD20; FcγRIIIA; Fc γRIIIA; Fcγ RIIIA; Fc γ RIIIA
Accession	P08637-1
Species	Human
Biotinylated	yes
Amino Acid Range	Gly17-Gln208(F176)
Molecular Weight	The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 48-58 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Anti-Fc gamma RIIIA Antibody, hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human Fc gamma RIIIA (F176), His-Avi Tag with the EC50 of 18.9ng/ml determined by ELISA (QC Test). The affinity constant of 0.14uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Biotinylated Human Fc gamma RIIIA (F176), His Tag captured on CM5 Chip via Anti-His Antibody can bind Rituximab, hFc Tag with an affinity constant of 0.14uM as determined in SPR assay (Biacore T200).
	Immobilized Anti-Fc gamma RIIIA Antibody, hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human Fc gamma RIIIA (F176), His-Avi Tag with the EC50 of 18.9ng/ml determined by ELISA (QC Test).
	Biotinylated Human Fc gamma RIIIA (F176) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human Fc gamma RIIIA (F176) is greater than 95% as determined by SEC-HPLC.