Biotinylated Human Fc gamma RIIIA/CD16a (V176) Protein (FCR-HM43AB)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Anti-Fc gamma RIIIA Antibody, hFc Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human Fc gamma RIIIA (V176) , His Tag with the EC50 of 28.2 ng/ml determined by ELISA (QC Test). The affinity constant of 0.539 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gly17-Gln208(V176)
Catalog #FCR-HM43AB
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Product Description
 Recombinant Biotinylated Human Fc gamma RIIIA/CD16a (V176) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus; It contains Gly17-Gln208(V176).
Product Background
 The Human Fc gamma RIIIA/CD16a Protein is a receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.
Product Category
 Recombinant Protein
Target
 Fc gamma RIIIA/CD16a (V176)
Synonyms
 CD16FCRIIIA; Fc fragment of IgG; Fc gamma RIIIA; Fc-gamma RIII-alpha; FCGR3; FCGR3A; FCGRIII; FcgRIIIA; FcR-10; CD16A; FCG3; IGFR3; FcRIIIa; FcRIII; CD16; IMD20
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Gly17-Gln208(V176)
Molecular Weight
 The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Anti-Fc gamma RIIIA Antibody, hFc Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human Fc gamma RIIIA (V176) , His Tag with the EC50 of 28.2 ng/ml determined by ELISA (QC Test). The affinity constant of 0.539 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His-Avi
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Rituximab captured on CM5 Chip via Protein A can bind Biotinylated Human Fc gamma RIIIA (V176) , His-Avi Tag with an affinity constant of 0.539 uM as determined in SPR assay (Biacore T200).
Immobilized Anti-Fc gamma RIIIA Antibody, hFc Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human Fc gamma RIIIA (V176) , His Tag with the EC50 of 28.2 ng/ml determined by ELISA (QC Test).
Biotinylated Human Fc gamma RIIIA (V176) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human Fc gamma RIIIA (V176) is greater than 95% as determined by SEC-HPLC.

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