Biotinylated Human Fc gamma RIIIA/CD16a (V176) Protein (FCR-HM43AB)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Anti-Fc gamma RIIIA Antibody, hFc Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human Fc gamma RIIIA (V176) , His Tag with the EC50 of 9.8 ng/ml determined by ELISA (QC Test). The affinity constant of 0.539 uM as determined in SPR assay (Biacore T200).
Amino Acid Range: Gly17-Gln208 (V176)

Catalog #FCR-HM43AB

Price

$577⁵⁰

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Biotinylated Human Fc gamma RIIIA/CD16a (V176) Protein (FCR-HM43AB) Summary

Product Description	Recombinant Biotinylated Human Fc gamma RIIIA/CD16a (V176) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus; It contains Gly17-Gln208 (V176).
Product Background	The Human Fc gamma RIIIA/CD16a Protein is a receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.

Protein	Fc gamma RIIIA/CD16a
Synonyms	Fc gamma RIIIA; Fc gamma RIIIA (V176); FCGR3; FCGR3A; FCGRIII; FcgRIIIA; FcR-10; CD16A; FCG3; IGFR3; FcRIIIa; FcRIII; CD16; IMD20; FcγRIIIA; Fc γRIIIA; Fcγ RIIIA; Fc γ RIIIA;FcR10;Fc gRIIIA; Fcg RIIIA; Fc g RIIIA
Accession	AAH17865
Species	Human
Biotinylated	yes
Amino Acid Range	Gly17-Gln208 (V176)
Molecular Weight	The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Anti-Fc gamma RIIIA Antibody, hFc Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human Fc gamma RIIIA (V176) , His Tag with the EC50 of 9.8 ng/ml determined by ELISA (QC Test). The affinity constant of 0.539 uM as determined in SPR assay (Biacore T200).
Product Tag	C-His-Avi
Fluorophore	Biotin-labeled
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Rituximab captured on CM5 Chip via Protein A can bind Biotinylated Human Fc gamma RIIIA (V176) , His-Avi Tag with an affinity constant of 0.539 uM as determined in SPR assay (Biacore T200).
	Immobilized Anti-Fc gamma RIIIA Antibody, hFc Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human Fc gamma RIIIA (V176) , His Tag with the EC50 of 9.8 ng/ml determined by ELISA (QC Test).
	Biotinylated Human Fc gamma RIIIA (V176) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human Fc gamma RIIIA (V176) is greater than 95% as determined by SEC-HPLC.