Product Description |
Recombinant Biotinylated Human Fc gamma RIIIB/CD16b (NA1) Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Gly17-Ser200. |
Product Background |
Human Fc gamma RIIIB/CD16b Protein is a receptor for the Fc region of immunoglobulins gamma. Low affinity receptor. Binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. May serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils. |
Product Category |
Recombinant Protein |
Protein |
Fc gamma RIIIB/CD16b (NA1) |
Synonyms |
CD16; Fc gamma RIIIB; FCG3; FCGR3B; FcgRIIIB; FCRIIIB; IGFR3; CD16b (NA2); CD16B; FCG3B; FCG3; FCGR3; FCR-10; FcγRIIIB; Fc γRIIIB; Fcγ RIIIB; Fc γ RIIIB |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Gly17-Ser200 |
Molecular Weight |
The protein has a predicted MW of 23.81 kDa. Due to glycosylation, the protein migrates to 43-53 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 8.00 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His-Avi |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Biotinylated Human Fc gamma RIIIB (NA1), His Tag captured on CM5 Chip via anti-his antibody can bind Rituximab with an affinity constant of 8.00 uM as determined in SPR assay (Biacore T200). |
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Biotinylated Human Fc gamma RIIIB (NA1) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human Fc gamma RIIIB (NA1) is greater than 95% as determined by SEC-HPLC. |
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