Name: Biotinylated Human Fc gamma RIIIB/CD16b (NA1) Protein (FCR-HM41BB)
Brand: KactusBio
SKU: FCR-HM41BB-100UG
Price: 577.5 USD
Availability: InStock

HPLC chromatogram showing main peak at 9.097 minutes and secondary peak at 11.350 minutes, indicating recombinant protein purity with minor variant or impurity

Biotinylated Human Fc gamma RIIIB/CD16b (NA1) Protein (FCR-HM41BB)

Biotinylated Human Fc gamma RIIIB (NA1), His Tag captured on CM5 Chip via anti-his antibody can bind Rituximab with an affinity constant of 8.00 uM as determined in SPR assay (Biacore T200).

Biotinylated Human Fc gamma RIIIB (NA1) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human Fc gamma RIIIB (NA1) is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 8.00 uM as determined in SPR assay (Biacore T200).
Amino Acid Range: Gly17-Ser200

Catalog #FCR-HM41BB

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Human Fc gamma RIIIB/CD16b (NA1) Protein (FCR-HM41BB) Summary

Product Description	Recombinant Biotinylated Human Fc gamma RIIIB/CD16b (NA1) Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Gly17-Ser200.
Product Background	Human Fc gamma RIIIB/CD16b Protein is a receptor for the Fc region of immunoglobulins gamma. Low affinity receptor. Binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. May serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils.
Product Category	Recombinant Protein / Antibody discovery / Fc receptors

Biotinylated Human Fc gamma RIIIB/CD16b (NA1) Protein (FCR-HM41BB) Specifications/Details

Protein	Fc gamma RIIIB/CD16b
Synonyms	CD16; Fc gamma RIIIB; FCG3; FCGR3B; FcgRIIIB; FCRIIIB; IGFR3; CD16b (NA2); CD16B; FCG3B; FCG3; FCGR3; FCR-10; FcγRIIIB; Fc γRIIIB; Fcγ RIIIB; Fc γ RIIIB
Accession	AAA35881.1
Species	Human
Biotinylated	yes
Amino Acid Range	Gly17-Ser200
Molecular Weight	The protein has a predicted MW of 23.81 kDa. Due to glycosylation, the protein migrates to 43-53 kDa based on Bis-Tris PAGE result.
Activity	The affinity constant of 8.00 uM as determined in SPR assay (Biacore T200).
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Biotinylated Human Fc gamma RIIIB (NA1), His Tag captured on CM5 Chip via anti-his antibody can bind Rituximab with an affinity constant of 8.00 uM as determined in SPR assay (Biacore T200).
	Biotinylated Human Fc gamma RIIIB (NA1) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human Fc gamma RIIIB (NA1) is greater than 95% as determined by SEC-HPLC.