Biotinylated Human Fc gamma RIIIB/CD16b (NA1) Protein (FCR-HM41BB)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: The affinity constant of 8.00 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gly17-Ser200

Catalog: FCR-HM41BB

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Product Description Recombinant Biotinylated Human Fc gamma RIIIB/CD16b (NA1) Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Gly17-Ser200.

Product Background

Human Fc gamma RIIIB/CD16b Protein is a receptor for the Fc region of immunoglobulins gamma. Low affinity receptor. Binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. May serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils.

Product Category

Recombinant Protein

Target

Fc gamma RIIIB/CD16b (NA1)
Accession

Species

Human

Amino Acid Range

Gly17-Ser200
Molecular Weight The protein has a predicted MW of 23.81 kDa. Due to glycosylation, the protein migrates to 43-53 kDa based on Tris-Bis PAGE result.

Activity

The affinity constant of 8.00 uM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Biotinylated Human Fc gamma RIIIB (NA1), His Tag captured on CM5 Chip via anti-his antibody can bind Rituximab with an affinity constant of 8.00 uM as determined in SPR assay (Biacore T200).
The purity of Biotinylated Human Fc gamma RIIIB (NA1) is greater than 95% as determined by SEC-HPLC.
Biotinylated Human Fc gamma RIIIB (NA1) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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