Name: Biotinylated Human FcRH5/FcRL5 Protein (Primary Amine Labeling) (FCR-HM101B)
Brand: KactusBio
SKU: FCR-HM101B-100UG
Price: 660.0 USD
Availability: InStock

Biotinylated Human FcRH5/FcRL5 Protein (Primary Amine Labeling) (FCR-HM101B)

Immobilized Anti-FcRH5 Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FcRH5, His Tag with the EC50 of 35.6ng/ml determined by ELISA (QC Test).

Biotinylated Human FcRH5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Loaded Anti-FcRH5 Antibody, hFc Tag on ProA-Biosensor can bind Biotinylated Human FcRH5, His Tag with an affinity constant of 12.60 nM as determined in BLI assay (Gator® Prime).

The purity of Biotinylated Human FcRH5 is greater than 90% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: Immobilized Anti-FcRH5 Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FcRH5, His Tag with the EC50 of 35.6ng/ml determined by ELISA (QC Test).
Amino Acid Range: Gln16-Gly851

Catalog #FCR-HM101B

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Human FcRH5/FcRL5 Protein (Primary Amine Labeling) (FCR-HM101B) Summary

Product Description	Recombinant Biotinylated Human FcRH5/FcRL5 Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus.It contains Gln16-Gly851.
Product Background	FcRH5 is a cell surface marker enriched on malignant plasma cells when compared to other hematologic malignancies and normal tissues. DFRF4539A is an anti-FcRH5 antibody-drug conjugated to monomethyl auristatin E (MMAE), a potent anti-mitotic agent.
Product Category	Recombinant Protein / Antibody discovery / ADC targets

Biotinylated Human FcRH5/FcRL5 Protein (Primary Amine Labeling) (FCR-HM101B) Specifications/Details

Protein	FcRH5/FcRL5
Synonyms	FcR-like protein 5; FcRH5; FcRL5; BXMAS1; BXMAS1MGC119592; CD307; CD307e; DKFZp667E2019; DKFZp667F216; FCRH5MGC119593; FLJ00333; FLJ00397; IFGP5; IRTA2
Accession	Q96RD9-1
Species	Human
Biotinylated	yes
Amino Acid Range	Gln16-Gly851
Molecular Weight	The protein has a predicted MW of 92.38 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Anti-FcRH5 Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FcRH5, His Tag with the EC50 of 35.6ng/ml determined by ELISA (QC Test).
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS, 100mM Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Anti-FcRH5 Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FcRH5, His Tag with the EC50 of 35.6ng/ml determined by ELISA (QC Test).
	Biotinylated Human FcRH5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Anti-FcRH5 Antibody, hFc Tag on ProA-Biosensor can bind Biotinylated Human FcRH5, His Tag with an affinity constant of 12.60 nM as determined in BLI assay (Gator® Prime).
	The purity of Biotinylated Human FcRH5 is greater than 90% as determined by SEC-HPLC.