Biotinylated Human FcRH5/FcRL5 Protein (Primary Amine Labeling) (FCR-HM101B)
Biotinylated Human FcRH5/FcRL5 Protein (Primary Amine Labeling) (FCR-HM101B)
Biotinylated Human FcRH5/FcRL5 Protein (Primary Amine Labeling) (FCR-HM101B)
Biotinylated Human FcRH5/FcRL5 Protein (Primary Amine Labeling) (FCR-HM101B)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Anti-FcRH5 Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FcRH5, His Tag with the EC50 of 35.6ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Gln16-Gly851
Catalog #FCR-HM101B
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Product Description
 Recombinant Biotinylated Human FcRH5/FcRL5 Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus.It contains Gln16-Gly851.
Product Background
 FcRH5 is a cell surface marker enriched on malignant plasma cells when compared to other hematologic malignancies and normal tissues. DFRF4539A is an anti-FcRH5 antibody-drug conjugated to monomethyl auristatin E (MMAE), a potent anti-mitotic agent.
Product Category
 Recombinant Protein
Target
 FcRH5/FcRL5
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Gln16-Gly851
Molecular Weight
 The protein has a predicted MW of 92.38 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Anti-FcRH5 Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FcRH5, His Tag with the EC50 of 35.6ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS, 100mM Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-FcRH5 Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FcRH5, His Tag with the EC50 of 35.6ng/ml determined by ELISA (QC Test).
Biotinylated Human FcRH5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human FcRH5 is greater than 90% as determined by SEC-HPLC.

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