Biotinylated Human FGF-7/KGF Protein (KGF-HE401B)
Biotinylated Human FGF-7/KGF Protein (KGF-HE401B)
  • Express System: E.coli
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human FGFR2 beta (IIIb), hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FGF-7, His-Avi Tag with the EC50 of 26.7ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Cys32-Thr194
Catalog #KGF-HE401B
Regular price $577.50
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Biotinylated Human FGF-7/KGF Protein (KGF-HE401B) Summary

Product Description

Recombinant Biotinylated Human FGF-7/KGF Protein is expressed from E.coli with His tag and Avi tag at the N-terminus.It contains Cys32-Thr194.

Product Background

The expression patterns of mRNAs encoding Fibroblast Growth Factor-7 (FGF-7) and its high affinity receptor suggested that FGF-7 signaling may play a role in regulating ureteric bud growth.t Results of these studies demonstrate that the developing ureteric bud and mature collecting system of FGF-7-null kidneys is markedly smaller than wild type. FGF-7 levels modulate the extent of ureteric bud growth during development and the number of nephrons that eventually form in the kidney.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Biotinylated Human FGF-7/KGF Protein (KGF-HE401B) Specifications/Details

Protein
FGF-7/KGF
Synonyms
FGF-7; HBGF-7; KGF
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Cys32-Thr194
Molecular Weight
The protein has a predicted MW of 21.79 kDa. The protein migrates to 25-28 kDa based on Tris-Bis PAGE result.
Activity
Immobilized Human FGFR2 beta (IIIb), hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FGF-7, His-Avi Tag with the EC50 of 26.7ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

N-His-Avi
Expression System
E.coli
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human FGFR2 beta (IIIb), hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FGF-7, His-Avi Tag with the EC50 of 26.7ng/ml determined by ELISA (QC Test). Immobilized Human FGFR2 beta (IIIb), hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FGF-7, His-Avi Tag with the EC50 of 26.7ng/ml determined by ELISA (QC Test).
Biotinylated Human FGF-7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human FGF-7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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