Name: Biotinylated Human FGF21 Protein (FGF-HM621B)
Brand: KactusBio
SKU: FGF-HM621B-100UG
Price: 577.5 USD
Availability: InStock

ELISA binding curve showing interaction between biotinylated human FGF21 and human beta klotho in a dose-dependent manner.

Biotinylated Human FGF21 Protein (FGF-HM621B)

Immobilized Biotinylated Human FGF21, mFc Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human Beta Klotho, His Tag with the EC50 of 0.71ug/ml determined by ELISA.

Biotinylated Human FGF21 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human FGF21 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: N-mFc-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human FGF21, mFc Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human Beta Klotho, His Tag with the EC50 of 0.71ug/ml determined by ELISA. See testing image for detail.
Amino Acid Range: His29-Ser209

Catalog #FGF-HM621B

Size

100UG

500UG

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Biotinylated Human FGF21 Protein (FGF-HM621B) Summary

Product Description	Recombinant Biotinylated Human FGF21 Protein is expressed from HEK293 with mFc (IgG1) tag and Avi tag at the N-Terminus.It contains His29-Ser209.
Product Background	Fibroblast growth factor 21 (FGF21) is a peptide hormone that is synthesized by several organs and regulates energy homeostasis. Excitement surrounding this relatively recently identified hormone is based on the documented metabolic beneficial effects of FGF21, which include weight loss and improved glycemia.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Biotinylated Human FGF21 Protein (FGF-HM621B) Specifications/Details

Protein	FGF21
Synonyms	UNQ3115; PRO10196; FGF-21; FGF21
Accession	Q9NSA1
Species	Human
Biotinylated	yes
Amino Acid Range	His29-Ser209
Molecular Weight	The protein has a predicted MW of 46.9 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human FGF21, mFc Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human Beta Klotho, His Tag with the EC50 of 0.71ug/ml determined by ELISA. See testing image for detail.
Product Tag	N-mFc-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Human FGF21, mFc Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human Beta Klotho, His Tag with the EC50 of 0.71ug/ml determined by ELISA.
	Biotinylated Human FGF21 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human FGF21 is greater than 95% as determined by SEC-HPLC.