Biotinylated Human FGFR2 alpha (IIIb) Protein (FGF-HM4ABB)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Anti-FGFR2 alpha (IIIb) Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human FGFR2 alpha (IIIb), His Tag with the EC50 of 18.7ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Arg22-Glu378

Catalog: FGF-HM4ABB

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Product Description Recombinant Biotinylated Human FGFR2 alpha (IIIb) Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.; It contains Arg22-Glu378.
Product Category Recombinant Protein
Target FGFR2 alpha (IIIb)
Synonyms FGF R2a; FGFR2 alpha; KGFR; CD332; BBDS; BEK; BFR-1; CEK3; CFD1; ECT1; JWS; K-SAM; KGFR; TK14; TK25; FLJ98662
Gene/Protein ID P21802-3
Species Human
Exact Sequence Arg22-Glu378
Molecular Weight The protein has a predicted MW of 42.5 kDa. Due to glycosylation, the protein migrates to 70-110 kDa based on Tris-Bis PAGE result.
Activity Immobilized Anti-FGFR2 alpha (IIIb) Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human FGFR2 alpha (IIIb), His Tag with the EC50 of 18.7ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-FGFR2 alpha (IIIb) Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human FGFR2 alpha (IIIb), His Tag with the EC50 of 18.7ng/ml determined by ELISA.
The purity of Biotinylated Human FGFR2 alpha (IIIb) is greater than 95% as determined by SEC-HPLC.
Biotinylated Human FGFR2 alpha (IIIb) on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

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