Biotinylated Human FGFR2 alpha (IIIb) Protein (FGF-HM4ABB)
Biotinylated Human FGFR2 alpha (IIIb) Protein (FGF-HM4ABB)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Anti-FGFR2 alpha (IIIb) Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FGFR2 alpha (IIIb) , His Tag with the EC50 of 20.3ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Arg22-Glu378
Catalog #FGF-HM4ABB
Regular price $525.00
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Product Description

Recombinant Biotinylated Human FGFR2 alpha (IIIb) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Arg22-Glu378.

Product Background

Four distinct genes encoding closely related FGF receptors, FGF R1 - 4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Multiple forms of FGF R1 - 3 are generated by alternative splicing of the mRNAs.

Product Category

Recombinant Protein / Antibody discovery

Protein

FGFR2 alpha (IIIb)

Synonyms

FGF R2a; FGFR2 alpha; KGFR; CD332; BBDS; BEK; BFR-1; CEK3; CFD1; ECT1; JWS; K-SAM; KGFR; TK14; TK25; FLJ98662

Accession

Species

Human

Biotinylated

yes

Amino Acid Range

Arg22-Glu378

Molecular Weight

The protein has a predicted MW of 42.5 kDa. Due to glycosylation, the protein migrates to 70-110 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Anti-FGFR2 alpha (IIIb) Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FGFR2 alpha (IIIb) , His Tag with the EC50 of 20.3ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Anti-FGFR2 alpha (IIIb) Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FGFR2 alpha (IIIb) , His Tag with the EC50 of 20.3ng/ml determined by ELISA. Immobilized Anti-FGFR2 alpha (IIIb) Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FGFR2 alpha (IIIb) , His Tag with the EC50 of 20.3ng/ml determined by ELISA.
Biotinylated Human FGFR2 alpha (IIIb) on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. Biotinylated Human FGFR2 alpha (IIIb) on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Biotinylated Human FGFR2 alpha (IIIb) is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human FGFR2 alpha (IIIb) is greater than 95% as determined by SEC-HPLC.

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