Biotinylated Human FGFR2 alpha (IIIb) Protein (FGF-HM4ABB)
Biotinylated Human FGFR2 alpha (IIIb) Protein (FGF-HM4ABB)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human FGFR2 alpha (IIIb), His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-FGFR2 (IIIb) Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Arg22-Glu378
Catalog #FGF-HM4ABB
Regular price $577.50
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Biotinylated Human FGFR2 alpha (IIIb) Protein (FGF-HM4ABB) Summary

Product Description

Recombinant Biotinylated Human FGFR2 alpha (IIIb) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Arg22-Glu378.

Product Background

Four distinct genes encoding closely related FGF receptors, FGF R1 - 4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Multiple forms of FGF R1 - 3 are generated by alternative splicing of the mRNAs.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Biotinylated Human FGFR2 alpha (IIIb) Protein (FGF-HM4ABB) Specifications/Details

Protein
FGFR2 alpha (IIIb)
Synonyms
FGF R2a; FGFR2 alpha; KGFR; CD332; BBDS; BEK; BFR-1; CEK3; CFD1; ECT1; JWS; K-SAM; KGFR; TK14; TK25; FLJ98662
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Arg22-Glu378
Molecular Weight
The protein has a predicted MW of 42.5 kDa. Due to glycosylation, the protein migrates to 70-110 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Human FGFR2 alpha (IIIb), His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-FGFR2 (IIIb) Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human FGFR2 alpha (IIIb), His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-FGFR2 (IIIb) Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA. Immobilized Biotinylated Human FGFR2 alpha (IIIb), His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-FGFR2 (IIIb) Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA.
Biotinylated Human FGFR2 alpha (IIIb) on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. Biotinylated Human FGFR2 alpha (IIIb) on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Biotinylated Human FGFR2 alpha (IIIb) is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human FGFR2 alpha (IIIb) is greater than 95% as determined by SEC-HPLC.
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