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Biotinylated Human FGFR2 alpha (IIIb) Protein, Ultra Low Endotoxin (FGF-HM4ABB-UL) Summary
Product Description |
Recombinant Biotinylated Human FGFR2 alpha (IIIb) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Arg22-Glu378. |
Product Background |
Four distinct genes encoding closely related FGF receptors, FGF R1 - 4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Multiple forms of FGF R1 - 3 are generated by alternative splicing of the mRNAs. |
Product Category |
Recombinant Protein / Antibody discovery / ADC targets |
Biotinylated Human FGFR2 alpha (IIIb) Protein, Ultra Low Endotoxin (FGF-HM4ABB-UL) Specifications/Details
Protein |
FGFR2 alpha (IIIb) |
Synonyms |
FGF R2a; FGFR2 alpha; KGFR; CD332; BBDS; BEK; BFR-1; CEK3; CFD1; ECT1; JWS; K-SAM; KGFR; TK14; TK25; FLJ98662 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Arg22-Glu378 |
Molecular Weight |
The protein has a predicted MW of 42.5 kDa. Due to glycosylation, the protein migrates to 70-110 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human FGFR2 alpha (IIIb), His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-FGFR2 (IIIb) Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA. |
Product Tag |
C-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Biotinylated Human FGFR2 alpha (IIIb), His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-FGFR2 (IIIb) Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA. |
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Biotinylated Human FGFR2 alpha (IIIb) on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. |
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The purity of Biotinylated Human FGFR2 alpha (IIIb) is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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