Product Description |
Recombinant Biotinylated Human FGFR2 beta (IIIb) Domain Protein is expressed from HEK293 with Avi tag at the C-Terminus.It contains Pro253-Glu378. |
Product Background |
Four distinct genes encoding closely related FGF receptors, FGF R1 - 4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Multiple forms of FGF R1 - 3 are generated by alternative splicing of the mRNAs. A frequent splicing event involving FGF R1 and 2 results in receptors containing all three Ig domains, referred to as the alpha isoform, or only IgII and IgIII, referred to as the beta isoform. |
Product Category |
Recombinant Protein |
Target |
FGFR2 beta (IIIb) Domain |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Pro253-Glu378 |
Molecular Weight |
The protein has a predicted MW of 15.49 kDa. Due to glycosylation, the protein migrates to 32-50 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human FGFR2 beta (IIIb) Domain, Avi Tag at 0.5 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for Anti-FGFR2 (IIIb) Antibody, hFc Tag with the EC50 of 4.8 ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-Avi |
Express System |
HEK293 |
Purity |
>95% as determined by Bis-Tris PAGE;>90% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Biotinylated Human FGFR2 beta (IIIb) Domain, Avi Tag at 0.5 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for Anti-FGFR2 (IIIb) Antibody, hFc Tag with the EC50 of 4.8 ng/ml determined by ELISA. |
|
|
Biotinylated Human FGFR2 beta (IIIb) Domain on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Biotinylated Human FGFR2 beta (IIIb) Domain is greater than 90% as determined by SEC-HPLC. |
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