Biotinylated Human FGFR2 beta (IIIb) Domain Protein (FGF-HM4BDB)
Biotinylated Human FGFR2 beta (IIIb) Domain Protein (FGF-HM4BDB)
Biotinylated Human FGFR2 beta (IIIb) Domain Protein (FGF-HM4BDB)
Biotinylated Human FGFR2 beta (IIIb) Domain Protein (FGF-HM4BDB)
  • Express System: HEK293
  • Product Tag: C-Avi
  • Purity: >95% as determined by Bis-Tris PAGE;>90% as determined by HPLC
  • Activity: Immobilized Biotinylated Human FGFR2 beta (IIIb) Domain, Avi Tag at 0.5 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for Anti-FGFR2 (IIIb) Antibody, hFc Tag with the EC50 of 1.6 ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Pro253-Glu378
Catalog #FGF-HM4BDB
Regular price $525.00
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Product Description

Recombinant Biotinylated Human FGFR2 beta (IIIb) Domain Protein is expressed from HEK293 with Avi tag at the C-Terminus.It contains Pro253-Glu378.

Product Background

Four distinct genes encoding closely related FGF receptors, FGF R1 - 4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Multiple forms of FGF R1 - 3 are generated by alternative splicing of the mRNAs. A frequent splicing event involving FGF R1 and 2 results in receptors containing all three Ig domains, referred to as the alpha isoform, or only IgII and IgIII, referred to as the beta isoform.

Product Category

Recombinant Protein / Antibody discovery

Protein

FGFR2 beta (IIIb) Domain

Synonyms

Fibroblast growth factor receptor 2;FGFR-2;KGFR;K-sam;Keratinocyte growth factor receptor;CD332;BEK;KSAM

Accession

Species

Human

Biotinylated

yes

Amino Acid Range

Pro253-Glu378

Molecular Weight

The protein has a predicted MW of 15.49 kDa. Due to glycosylation, the protein migrates to 32-50 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Biotinylated Human FGFR2 beta (IIIb) Domain, Avi Tag at 0.5 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for Anti-FGFR2 (IIIb) Antibody, hFc Tag with the EC50 of 1.6 ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-Avi

Expression System

HEK293

Purity

>95% as determined by Bis-Tris PAGE;>90% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human FGFR2 beta (IIIb) Domain, Avi Tag at 0.5 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for Anti-FGFR2 (IIIb) Antibody, hFc Tag with the EC50 of 1.6 ng/ml determined by ELISA (QC Test). Immobilized Biotinylated Human FGFR2 beta (IIIb) Domain, Avi Tag at 0.5 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for Anti-FGFR2 (IIIb) Antibody, hFc Tag with the EC50 of 1.6 ng/ml determined by ELISA (QC Test).
Biotinylated Human FGFR2 beta (IIIb) Domain on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human FGFR2 beta (IIIb) Domain on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human FGFR2 beta (IIIb) Domain is greater than 90% as determined by SEC-HPLC. The purity of Biotinylated Human FGFR2 beta (IIIb) Domain is greater than 90% as determined by SEC-HPLC.

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