Biotinylated Human FGFR3 alpha (IIIb) Protein (FGF-HM43BB)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Human FGFR3 alpha (lllb) at 1_g/ml (100_l/Well) on the plate. Dose response curve for Anti-FGFR3 Antibody, hFc Tag with the EC50 of 11.9ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Glu23-Gly377

Catalog: FGF-HM43BB

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Product Description Recombinant Biotinylated Human FGFR3 (IIIb) Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Glu23-Gly377.
Product Category Recombinant Protein
Target FGFR3 alpha (IIIb)
Synonyms ACH; CD333; CEK; CEK2; EC 2.7.10; FGF R3; FGFR3; HSFGFR3EX; JTK4
Gene/Protein ID P22607-2
Species Human
Exact Sequence Glu23-Gly377
Molecular Weight The protein has a predicted MW of 41.4 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Tris-Bis PAGE result.
Activity Immobilized Biotinylated Human FGFR3 alpha (lllb) at 1_g/ml (100_l/Well) on the plate. Dose response curve for Anti-FGFR3 Antibody, hFc Tag with the EC50 of 11.9ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per _g by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22_m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 _g/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80¡C for 12 months, 4¡C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human FGFR3 alpha (lllb) at 1_g/ml (100_l/Well) on the plate. Dose response curve for Anti-FGFR3 Antibody, hFc Tag with the EC50 of 11.9ng/ml determined by ELISA.
The purity of Biotinylated Human FGFR3 alpha (IIIb) is greater than 95% as determined by SEC-HPLC.
Biotinylated Human FGFR3 alpha (IIIb) on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

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