Biotinylated Human FGFR3 alpha (IIIc) Protein (FGF-HM43CB)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Human FGFR3 alpha  (IIIc) at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-FGFR3 Antibody, hFc Tag with the EC50 of 9.7ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Glu23-Gly375

Catalog: FGF-HM43CB

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Product Description Recombinant Biotinylated Human FGFR3 alpha (IIIc) Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Glu23-Gly375.
Product Category Recombinant Protein
Target FGFR3 alpha (IIIc)
Synonyms ACH; CD333; CEK; CEK2; EC 2.7.10; FGF R3; FGFR3; HSFGFR3EX; JTK4
Gene/Protein ID P22607
Species Human
Exact Sequence Glu23-Gly375
Molecular Weight The protein has a predicted MW of 41.1 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Tris-Bis PAGE result.
Activity Immobilized Biotinylated Human FGFR3 alpha  (IIIc) at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-FGFR3 Antibody, hFc Tag with the EC50 of 9.7ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human FGFR3 alpha  (IIIc) at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-FGFR3 Antibody, hFc Tag with the EC50 of 9.7ng/ml determined by ELISA.
The purity of Biotinylated Human FGFR3 alpha  (IIIc) is greater than 95% as determined by SEC-HPLC.
Biotinylated Human FGFR3 alpha  (IIIc) on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

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