Biotinylated Human FGFR3 beta (IIIb) Protein (FGF-HM4BBB) Summary
Product Description |
Recombinant Biotinylated Human FGFR3 beta (IIIb) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Asp127-Gly377. |
Product Background |
Four distinct genes encoding closely related FGF receptors, FGF R1-4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid‑box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain.FGFR3 is tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation and apoptosis. |
Product Category |
Recombinant Protein / Antibody discovery / ADC targets |
Biotinylated Human FGFR3 beta (IIIb) Protein (FGF-HM4BBB) Specifications/Details
Protein |
FGFR3 bata (IIIb) |
Synonyms |
ACH; CD333; CEK; CEK2; EC 2.7.10; FGF R3; FGFR3; HSFGFR3EX; JTK4 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Asp127-Gly377 |
Molecular Weight |
The protein has a predicted MW of 30.5 kDa. Due to glycosylation, the protein migrates to 40-55 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human FGFR3 beta (IIIb) , His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-FGFR3 Antibody, hFc Tag with the EC50 of 2.2ng/ml determined by ELISA. |
Product Tag |
C-His-Avi |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Biotinylated Human FGFR3 beta (IIIb) , His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-FGFR3 Antibody, hFc Tag with the EC50 of 2.2ng/ml determined by ELISA. |
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Biotinylated Human FGFR3 beta (IIIb) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human FGFR3 beta (IIIb) is greater than 95% as determined by SEC-HPLC. |
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