Biotinylated Human FGFR3 beta (IIIb) Protein (FGF-HM4BBB)
Biotinylated Human FGFR3 beta (IIIb) Protein (FGF-HM4BBB)
Biotinylated Human FGFR3 beta (IIIb) Protein (FGF-HM4BBB)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human FGFR3 beta (IIIb) , His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-FGFR3 Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Asp127-Gly377
Catalog #FGF-HM4BBB
Regular price $525.00
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Product Description
Recombinant Biotinylated Human FGFR3 beta (IIIb) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Asp127-Gly377.
Product Background
Four distinct genes encoding closely related FGF receptors, FGF R1-4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid‑box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain.FGFR3 is tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation and apoptosis.
Product Category
Recombinant Protein
Target
FGFR3 bata (IIIb)
Synonyms
ACH; CD333; CEK; CEK2; EC 2.7.10; FGF R3; FGFR3; HSFGFR3EX; JTK4
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Asp127-Gly377
Molecular Weight
The protein has a predicted MW of 30.5 kDa. Due to glycosylation, the protein migrates to 40-55 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Human FGFR3 beta (IIIb) , His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-FGFR3 Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human FGFR3 beta (IIIb) , His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-FGFR3 Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA.
Biotinylated Human FGFR3 beta (IIIb) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human FGFR3 beta (IIIb) is greater than 95% as determined by SEC-HPLC.
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