Biotinylated Human FGFR3 beta (IIIc) Protein (FGF-HM4BCB)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Anti-FGFR3 Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FGFR3 beta (IIIc) , His Tag with the EC50 of 37ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Asp127-Gly377

Catalog: FGF-HM4BCB

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Product Description Recombinant Biotinylated Human FGFR3 beta (IIIc) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Asp127-Gly377.
Product Category Recombinant Protein
Target FGFR3 beta (IIIc)
Synonyms ACH; CD333; CEK; CEK2; EC 2.7.10; FGF R3; FGFR3; HSFGFR3EX; JTK4
Gene/Protein ID P22607
Species Human
Exact Sequence Asp127-Gly377
Molecular Weight The protein has a predicted MW of 30.1 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
Activity Immobilized Anti-FGFR3 Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FGFR3 beta (IIIc) , His Tag with the EC50 of 37ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-FGFR3 Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FGFR3 beta (IIIc) , His Tag with the EC50 of 37ng/ml determined by ELISA.
The purity of Biotinylated Human FGFR3 beta (IIIc) is greater than 95% as determined by SEC-HPLC.
Biotinylated Human FGFR3 beta (IIIc) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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