Biotinylated Human FGL1 Protein (FGL-HM411B)
Biotinylated Human FGL1 Protein (FGL-HM411B)
Biotinylated Human FGL1 Protein (FGL-HM411B)
Biotinylated Human FGL1 Protein (FGL-HM411B)
  • Express System: HEK293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Biotinylated Human FGL1, His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human LAG3, hFc Tag with the EC50 of 0.19ug/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Asp64-Asn305
Catalog #FGL-HM411B
Regular price $525.00
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Product Description
Recombinant Biotinylated Human FGL1 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Asp64-Asn305.
Product Background
Fibrinogen-like protein 1 (FGL-1) is a protein that is structurally related to fibrinogen. In humans, FLG-1 is encoded by the FGL1 gene.Fibrinogen-like protein 1 is a member of the fibrinogen family of proteins, which also includes fibrinogen, fibrinogen-like protein 2, and clotting factors V, VIII, and XIII.Fibrinogen-like Protein 1 is a major immune inhibitory ligand of LAG-3.
Product Category
Recombinant Protein
Target
FGL1
Synonyms
FGL1; FGL-1; Hepassocin; HP-041; HFREP-1; LFIRE-1; HFREP1; Lag3 ligand
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Asp64-Asn305
Molecular Weight
The protein has a predicted MW of 31.9 kDa same as Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Human FGL1, His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human LAG3, hFc Tag with the EC50 of 0.19ug/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
N-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human FGL1, His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human LAG3, hFc Tag with the EC50 of 0.19ug/ml determined by ELISA (QC Test).
Biotinylated Human FGL1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%..

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