Name: Biotinylated Human FGL1 Protein (FGL-HM411B)
Brand: KactusBio
SKU: FGL-HM411B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human FGL1 Protein (FGL-HM411B)

Immobilized Biotinylated Human FGL1, His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human LAG3, hFc Tag with the EC50 of 0.19ug/ml determined by ELISA (QC Test).

Biotinylated Human FGL1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%..

Express System: HEK293
Product Tag: N-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Biotinylated Human FGL1, His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human LAG3, hFc Tag with the EC50 of 0.19ug/ml determined by ELISA (QC Test).
Amino Acid Range: Asp64-Asn305

Catalog #FGL-HM411B

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Human FGL1 Protein (FGL-HM411B) Summary

Product Description	Recombinant Biotinylated Human FGL1 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Asp64-Asn305.
Product Background	Fibrinogen-like protein 1 (FGL-1) is a protein that is structurally related to fibrinogen. In humans, FLG-1 is encoded by the FGL1 gene.Fibrinogen-like protein 1 is a member of the fibrinogen family of proteins, which also includes fibrinogen, fibrinogen-like protein 2, and clotting factors V, VIII, and XIII.Fibrinogen-like Protein 1 is a major immune inhibitory ligand of LAG-3.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Biotinylated Human FGL1 Protein (FGL-HM411B) Specifications/Details

Protein	FGL1
Synonyms	FGL1; FGL-1; Hepassocin; HP-041; HFREP-1; LFIRE-1; HFREP1; Lag3 ligand
Accession	Q08830
Species	Human
Biotinylated	yes
Amino Acid Range	Asp64-Asn305
Molecular Weight	The protein has a predicted MW of 31.9 kDa same as Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human FGL1, His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human LAG3, hFc Tag with the EC50 of 0.19ug/ml determined by ELISA (QC Test).
Product Tag	N-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Biotinylated Human FGL1, His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human LAG3, hFc Tag with the EC50 of 0.19ug/ml determined by ELISA (QC Test).
	Biotinylated Human FGL1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%..