Biotinylated Human FGL1 Protein (Primary Amine Labeling)  (FGL-HM211B)
Biotinylated Human FGL1 Protein (Primary Amine Labeling)  (FGL-HM211B)
  • Express System: HEK293
  • Product Tag: N-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Human LAG3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FGL1, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Asp64-Asn305
Catalog #FGL-HM211B
Regular price $525.00
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Product Description
Recombinant Biotinylated Human FGL1 Protein (Primary Amine Labeling) is expressed from HEK293 with hFc tag at the N-Terminus.It contains Asp64-Asn305.
Product Background
Fibrinogen-like protein 1 (FGL-1) is a protein that is structurally related to fibrinogen. In humans, FLG-1 is encoded by the FGL1 gene.Fibrinogen-like protein 1 is a member of the fibrinogen family of proteins, which also includes fibrinogen, fibrinogen-like protein 2, and clotting factors V, VIII, and XIII.Fibrinogen-like Protein 1 is a major immune inhibitory ligand of LAG-3.
Product Category
Recombinant Protein
Target
FGL1
Synonyms
FGL1; FGL-1; Hepassocin; HP-041; HFREP-1; LFIRE-1; HFREP1; Lag3 ligand
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Asp64-Asn305
Molecular Weight
The protein has a predicted MW of 54.8 kDa. Due to glycosylation, the protein migrates to 60-66 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human LAG3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FGL1, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA. See testing image for detail.
Product Tag
N-hFc
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22um filtered solution in 20mM PB, 250mM NaCl (pH 7.4).
Stability And Storage
Valid for 12 months from date of receipt when stored at -80°C.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human LAG3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FGL1, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA.
Biotinylated Human FGL1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human FGL1 is greater than 90% as determined by SEC-HPLC.

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