- Express System: HEK293
- Product Tag: N-hFc
- Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
- Activity: Immobilized Human LAG3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FGL1, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA. See testing image for detail.
- Amino Acid Range: Asp64-Asn305
Product Description |
Recombinant Biotinylated Human FGL1 Protein (Primary Amine Labeling) is expressed from HEK293 with hFc tag at the N-Terminus.It contains Asp64-Asn305. |
Product Background |
Fibrinogen-like protein 1 (FGL-1) is a protein that is structurally related to fibrinogen. In humans, FLG-1 is encoded by the FGL1 gene.Fibrinogen-like protein 1 is a member of the fibrinogen family of proteins, which also includes fibrinogen, fibrinogen-like protein 2, and clotting factors V, VIII, and XIII.Fibrinogen-like Protein 1 is a major immune inhibitory ligand of LAG-3. |
Product Category |
Recombinant Protein / Antibody discovery |
Protein |
FGL1 |
Synonyms |
FGL1; FGL-1; Hepassocin; HP-041; HFREP-1; LFIRE-1; HFREP1; Lag3 ligand |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Asp64-Asn305 |
Molecular Weight |
The protein has a predicted MW of 54.8 kDa. Due to glycosylation, the protein migrates to 60-66 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human LAG3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FGL1, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
N-hFc |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Formulation |
Supplied as 0.22um filtered solution in 20mM PB, 250mM NaCl (pH 7.4). |
Stability And Storage |
Valid for 12 months from date of receipt when stored at -80°C.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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