Biotinylated Human FGL2 Protein (FGL-HM612B)
Biotinylated Human FGL2 Protein (FGL-HM612B)
Biotinylated Human FGL2 Protein (FGL-HM612B)
  • Express System: HEK293
  • Product Tag: N-His-Avi,N-Flag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human FGL2, His Tag at 2ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-FGL2 Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Val205-Pro439
Catalog #FGL-HM612B
Regular price $577.50
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Biotinylated Human FGL2 Protein (FGL-HM612B) Summary

Product Description

Recombinant Biotinylated Human FGL2 Protein is expressed from HEK293 with His-Avi tag and Flag tag at the N-Terminus.It contains Val205-Pro439.

Product Background

Fibrinogen-like protein 2 (FGL2) is a member of the fibrinogen-like protein family and possesses important regulatory functions in both innate and adaptive immune responses. FGL2 is overexpressed in glioma, and its expression level is negatively associated with the prognosis of glioma patients.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Biotinylated Human FGL2 Protein (FGL-HM612B) Specifications/Details

Protein
FGL2
Synonyms
Fibroleukin; pT49; FGL2; fibrinogen-like 2;
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Val205-Pro439
Molecular Weight
The protein has a predicted MW of 31.2 kDa. Due to glycosylation, the protein migrates to 40-55 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Human FGL2, His Tag at 2ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-FGL2 Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

N-His-Avi,N-Flag
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human FGL2, His Tag at 2ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-FGL2 Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA (QC Test). Immobilized Biotinylated Human FGL2, His Tag at 2ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-FGL2 Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA (QC Test).
Biotinylated Human FGL2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human FGL2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human FGL2 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human FGL2 is greater than 95% as determined by SEC-HPLC.
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