Name: Biotinylated Human FLT3/Flk-2 Protein (FLT-HM430B)
Brand: KactusBio
SKU: FLT-HM430B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human FLT3/Flk-2 Protein (FLT-HM430B)

Immobilized Biotinylated Human FLT3, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human FLT3 Ligand, hFc Tag with the EC50 of 1.2ng/ml determined by ELISA (QC Test).

Biotinylated Human FLT3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human FLT3 is greater than 95% as determined by SEC-HPLC.

Express System: CHO
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human FLT3, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human FLT3 Ligand, hFc Tag with the EC50 of 1.2ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Asn27-Asn541

Catalog #FLT-HM430B

Size

100UG

500UG

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Biotinylated Human FLT3/Flk-2 Protein (FLT-HM430B) Summary

Product Description	Recombinant Biotinylated Human FLT3/Flk-2 Protein is expressed from CHO with His tag and Avi tag at the C-Terminus.It contains Asn27-Asn541.
Product Background	The Flt-3 (fms-like tyrosine kinase) receptor, also named Flk-2 (fetal liver kinase) and Stk-1(stem cell tyrosine kinase) is a member of the class III subfamily of receptor tyrosine kinases that also includes KIT, the receptor for SCF and FMS, the receptor for M-CSF. Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Biotinylated Human FLT3/Flk-2 Protein (FLT-HM430B) Specifications/Details

Protein	FLT3/Flk-2
Synonyms	CD135; FLK2; STK-1; FLT-3
Accession	P36888-1
Species	Human
Biotinylated	yes
Amino Acid Range	Asn27-Asn541
Molecular Weight	The protein has a predicted MW of 61.20 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human FLT3, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human FLT3 Ligand, hFc Tag with the EC50 of 1.2ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His-Avi
Expression System	CHO
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Human FLT3, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human FLT3 Ligand, hFc Tag with the EC50 of 1.2ng/ml determined by ELISA (QC Test).
	Biotinylated Human FLT3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human FLT3 is greater than 95% as determined by SEC-HPLC.