• Express System: HEK293
  • Product Tag: C-His-Twin Strep
  • Activity: Immobilized Biotinylated Human Frizzled-4 Nanodisc, His-Twin Strep Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Frizzled-4 Antibody, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA.
  • Amino Acid Range: Met1-Val537
Catalog #FZD-HM60NB
Price
Regular price $2,16600
Regular price Sale price $2,16600
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Size
Avantor VWR Fisher Scientific

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Biotinylated Human Frizzled-4/FZD4 Nanodisc (FZD-HM60NB) Summary

Product Description

 Recombinant Biotinylated Human Frizzled-4/FZD4 Nanodisc is expressed from HEK293 with Twin Strep tag and His tag at the C-terminus.It contains Met1-Val537.

Product Background

 Frizzled4 (FZD4) is a receptor for Norrin and WNT7A/B ligands, is expressed in endothelial cells (ECs), is required for blood-CNS-barrier function as well as CNS angiogenesis, and transduces β-catenin-dependent signaling. FZD4 associates with additional receptor complex components: Tetraspanin12 (TSPAN12) is a Norrin co-receptor that amplifies signaling in a ligand specific manner.

Biotinylated Human Frizzled-4/FZD4 Nanodisc (FZD-HM60NB) Specifications/Details

Protein
 Frizzled-4/FZD4
Synonyms
 Frizzled-4; Fz-4; hFz4; FzE4; CD344; FZD4; FZD4S; FEVR; GPCR; Fz4
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Met1-Val537
Molecular Weight
 The protein has a predicted MW of 72.40 kDa.
Activity
 Immobilized Biotinylated Human Frizzled-4 Nanodisc, His-Twin Strep Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Frizzled-4 Antibody, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA.
Product Tag
 C-His-Twin Strep
Fluorophore
 Biotin-labeled
Expression System
 HEK293
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
Stability And Storage
 Valid for 6 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Biotinylated Human Frizzled-4 Nanodisc, His-Twin Strep Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Frizzled-4 Antibody, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA. Immobilized Biotinylated Human Frizzled-4 Nanodisc, His-Twin Strep Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Frizzled-4 Antibody, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA.
Other Available Proteins:
Finding available proteins...