• Express System: HEK293
  • Product Tag: N-hFc (IgG1)-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Anti-Galectin 1 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Galectin 1, hFc-Avi Tag with the EC50 of 25.2ng/ml determined by ELISA.
  • Amino Acid Range: Ala2-Asp135
Catalog #GAL-HM501B
Price
Regular price $57800
Regular price Sale price $57800
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Human Galectin 1 Protein (GAL-HM501B) Summary

Product Description

Recombinant Biotinylated Human Galectin 1 Protein is expressed from HEK293 with hFc (IgG1) tag and Avi tag at the N-terminus.It contains Ala2-Asp135.

Product Background

Galectin 1(Gal-1), a β-galactoside binding mammalian lectin of 14KDa, is implicated in many signalling pathways, immune responses associated with cancer progression and immune disorders. Inhibition of human Gal-1 has been regarded as one of the potential therapeutic approaches for the treatment of cancer, as it plays a major role in tumour development and metastasis by modulating various biological functions viz. apoptosis, angiogenesis, migration, cell immune escape.

Biotinylated Human Galectin 1 Protein (GAL-HM501B) Specifications/Details

Protein
GAL1
Synonyms
Galectin-1; HLBP14; S-Lac lectin 1; LGALS1; GAL1; GBP; DKFZp686E23103; Galectin 1
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Ala2-Asp135
Molecular Weight
The protein has a predicted MW of 43.11 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Anti-Galectin 1 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Galectin 1, hFc-Avi Tag with the EC50 of 25.2ng/ml determined by ELISA.
Product Tag
N-hFc (IgG1)-Avi
Fluorophore
Biotin-labeled
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Anti-Galectin 1 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Galectin 1, hFc-Avi Tag with the EC50 of 25.2ng/ml determined by ELISA. Immobilized Anti-Galectin 1 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Galectin 1, hFc-Avi Tag with the EC50 of 25.2ng/ml determined by ELISA.
Biotinylated Human Galectin 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human Galectin 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human Galectin 1 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human Galectin 1 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...