Name: Biotinylated Human GARP&Latent TGF beta 1 Complex Protein (GAT-HM401B)
Brand: KactusBio
SKU: GAT-HM401B-100UG
Price: 742.5 USD
Availability: InStock

Biotinylated Human GARP&Latent TGF beta 1 Complex Protein (GAT-HM401B)

Immobilized Human ITGAV&ITGB6, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GARP&Latent TGF Beta 1 Complex, His Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test).

Immobilized Anti-GARP&Latent TGF beta 1 Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GARP&Latent TGF Beta 1 Complex, His Tag with the EC50 of 0.13ug/ml determined by ELISA.

Biotinylated Human GARP&Latent TGF beta 1 Complex on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinyated Human GARP&Latent TGF beta 1 Complex is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human ITGAV&ITGB6, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GARP&Latent TGF Beta 1 Complex, His Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: His20-Leu628(GARP) & Leu30-Ser390(Latent TGF beta 1)

Catalog #GAT-HM401B

Size

100UG

500UG

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Biotinylated Human GARP&Latent TGF beta 1 Complex Protein (GAT-HM401B) Summary

Product Description	Recombinant Biotinylated Human GARP&Latent TGF beta 1 Complex Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus of GARP.It contains His20-Leu628 (GARP) and Leu30-Ser390 (Latent TGF beta 1).
Product Background	GARP&Latent TGF Beta is a complex found on surface of many types of cells. In Tregs, GARP is involved in TCR-mediated activation of Latent TGF-β and thus promoting secretion and activation of TGF-β. Integrin αvβ8 on the surface of immune cells and other cells recognizes RGD in LAP, resulting in the release of mature TGF-β from the TGF-β&GARP complex.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Biotinylated Human GARP&Latent TGF beta 1 Complex Protein (GAT-HM401B) Specifications/Details

Protein	GARP&Latent TGF beta 1 Complex
Synonyms	LRRC32; GARP; LAP; TGF-beta-1; LRRC32&TGF-beta 1; LRRC32&TGFB1; TGF-β-1; LRRC32&TGF-β 1; GARP&Latent TGF Beta; GARP&Latent TGF Beta 1
Accession	Q14392(GARP)&P01137(Latent TGF beta 1)
Species	Human
Biotinylated	yes
Amino Acid Range	His20-Leu628(GARP) & Leu30-Ser390(Latent TGF beta 1)
Molecular Weight	The protein has a predicted MW of 70.3 kDa (GARP) and 41.4 kDa (Latent TGF beta 1). Due to glycosylation, the protein migrates to 73-78 kDa (GARP) and 13 kDa and 42-45 kDa (Latent TGF beta 1) based on Bis-Tris PAGE result.
Activity	Immobilized Human ITGAV&ITGB6, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GARP&Latent TGF Beta 1 Complex, His Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human ITGAV&ITGB6, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GARP&Latent TGF Beta 1 Complex, His Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test).
	Immobilized Anti-GARP&Latent TGF beta 1 Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GARP&Latent TGF Beta 1 Complex, His Tag with the EC50 of 0.13ug/ml determined by ELISA.
	Biotinylated Human GARP&Latent TGF beta 1 Complex on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinyated Human GARP&Latent TGF beta 1 Complex is greater than 95% as determined by SEC-HPLC.