Biotinylated Human GCGR/Glucagon receptor Nanodisc (GCR-HM10NB) Summary
Product Description |
Recombinant Biotinylated Human GCGR/Glucagon receptor Nanodisc is expressed from HEK293 with His tag at the C-terminus.It contains Met1-Phe477. |
Product Background |
The pancreatic hormone glucagon activates the glucagon receptor (GCGR), a class B seven-transmembrane G protein-coupled receptor that couples to the stimulatory heterotrimeric G protein and provokes PKA-dependent signaling cascades vital to hepatic glucose metabolism and islet insulin secretion. The GCGR expressed at the plasma membrane is constitutively ubiquitinated and upon agonist-activation, internalized GCGRs are deubiquitinated at early endosomes and recycled via Rab4-containing vesicles. |
Product Category |
Recombinant Protein / Antibody discovery / Multiple transmembrane proteins / Nanodisc |
Biotinylated Human GCGR/Glucagon receptor Nanodisc (GCR-HM10NB) Specifications/Details
Protein |
GCGR/Glucagon receptor |
Synonyms |
Glucagon receptor; GL-R; GCGR; Glucagon R |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Met1-Phe477 |
Molecular Weight |
The protein has a predicted MW of 66.9 kDa. |
Activity |
Immobilized Biotinylated Human GCGR Nanodisc, His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-GCGR Antibody, hFc Tag with the EC50 of 15.0ng/ml determined by ELISA (QC Test). The affinity constant of 5.02 nM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Formulation |
Supplied as 0.22 um filtered solution in PBS, 200mM L-arginine (pH 7.4). |
Stability And Storage |
Valid for 6 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Biotinylated Human GCGR Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Anti-GCGR Antibody, hFc Tag with an affinity constant of 5.02 nM as determined in SPR assay (Biacore T200). |
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Immobilized Biotinylated Human GCGR Nanodisc, His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-GCGR Antibody, hFc Tag with the EC50 of 15.0ng/ml determined by ELISA (QC Test). |
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Loaded Biotinylated Human GCGR Nanodisc, His Tag on Streptavidin -Biosensor can bind Anti-GCGR Antibody, hFc Tag with an affinity constant of 8.61 nM as determined in BLI assay (Gator® Prime). |
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