Name: Biotinylated Human GCGR/Glucagon receptor Protein-Nanodisc (GCR-HM10NB)
Brand: KactusBio
SKU: GCR-HM10NB-100UG
Price: 2165.9 USD
Availability: InStock

Biotinylated Human GCGR/Glucagon receptor Protein-Nanodisc (GCR-HM10NB)

Immobilized Biotinylated Human GCGR Nanodisc, His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-GCGR Antibody, hFc Tag with the EC50 of 15.0ng/ml determined by ELISA (QC Test).

Loaded Biotinylated Human GCGR Nanodisc, His Tag on Streptavidin -Biosensor can bind Anti-GCGR Antibody, hFc Tag with an affinity constant of 8.61 nM as determined in BLI assay (Gator® Prime).

Express System: HEK293
Product Tag: C-His
Activity: Immobilized Biotinylated Human GCGR Nanodisc, His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-GCGR Antibody, hFc Tag with the EC50 of 15.0ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Met1-Phe477

Catalog #GCR-HM10NB

Size

100UG

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Product Description	Recombinant Biotinylated Human GCGR/Glucagon receptor Protein-Nanodisc is expressed from HEK293 with His tag at the C-terminus.It contains Met1-Phe477.
Product Background	The pancreatic hormone glucagon activates the glucagon receptor (GCGR), a class B seven-transmembrane G protein-coupled receptor that couples to the stimulatory heterotrimeric G protein and provokes PKA-dependent signaling cascades vital to hepatic glucose metabolism and islet insulin secretion. The GCGR expressed at the plasma membrane is constitutively ubiquitinated and upon agonist-activation, internalized GCGRs are deubiquitinated at early endosomes and recycled via Rab4-containing vesicles.
Product Category	Recombinant Protein / Antibody discovery / Multiple transmembrane proteins / Nanodisc

Protein	GCGR/Glucagon receptor
Synonyms	Glucagon receptor; GL-R; GCGR; Glucagon R
Accession	P47871
Species	Human
Biotinylated	yes
Amino Acid Range	Met1-Phe477
Molecular Weight	The protein has a predicted MW of 66.9 kDa.
Activity	Immobilized Biotinylated Human GCGR Nanodisc, His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-GCGR Antibody, hFc Tag with the EC50 of 15.0ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in PBS, 200mM L-Arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
Stability And Storage	Valid for 6 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Human GCGR Nanodisc, His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-GCGR Antibody, hFc Tag with the EC50 of 15.0ng/ml determined by ELISA (QC Test).
	Loaded Biotinylated Human GCGR Nanodisc, His Tag on Streptavidin -Biosensor can bind Anti-GCGR Antibody, hFc Tag with an affinity constant of 8.61 nM as determined in BLI assay (Gator® Prime).