Biotinylated Human GCGR/Glucagon receptor Protein-Nanodisc (GCR-HM10NB)
  • Express System: HEK293
  • Product Tag: C-His
  • Activity: Immobilized Biotinylated Human GCGR Nanodisc, His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-GCGR Antibody, hFc Tag with the EC50 of 15.0ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Met1-Phe477
Catalog #GCR-HM10NB
Regular price $1,969.00
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Product Description

Recombinant Biotinylated Human GCGR/Glucagon receptor Protein-Nanodisc is expressed from HEK293 with His tag at the C-terminus.It contains Met1-Phe477.

Product Background

The pancreatic hormone glucagon activates the glucagon receptor (GCGR), a class B seven-transmembrane G protein-coupled receptor that couples to the stimulatory heterotrimeric G protein and provokes PKA-dependent signaling cascades vital to hepatic glucose metabolism and islet insulin secretion. The GCGR expressed at the plasma membrane is constitutively ubiquitinated and upon agonist-activation, internalized GCGRs are deubiquitinated at early endosomes and recycled via Rab4-containing vesicles.

Product Category

Recombinant Protein / Antibody discovery / Multiple transmembrane proteins / Nanodisc

Protein

GCGR/Glucagon receptor

Synonyms

Glucagon receptor; GL-R; GCGR; Glucagon R

Accession

Species

Human

Biotinylated

yes

Amino Acid Range

Met1-Phe477

Molecular Weight

The protein has a predicted MW of 66.9 kDa.

Activity

Immobilized Biotinylated Human GCGR Nanodisc, His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-GCGR Antibody, hFc Tag with the EC50 of 15.0ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His

Expression System

HEK293

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22 um filtered solution in PBS (pH 7.4). Notice: Not recommended for immunization.
Stability And Storage
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human GCGR Nanodisc, His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-GCGR Antibody, hFc Tag with the EC50 of 15.0ng/ml determined by ELISA (QC Test). Immobilized Biotinylated Human GCGR Nanodisc, His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-GCGR Antibody, hFc Tag with the EC50 of 15.0ng/ml determined by ELISA (QC Test).

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