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Biotinylated Human GCGR/Glucagon receptor Protein, Ultra Low Endotoxin (GCR-HM40RB-UL) Summary
Product Description |
Recombinant Biotinylated Human GCGR/Glucagon receptor Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Ala26-Lys136. |
Product Background |
The pancreatic hormone glucagon activates the glucagon receptor (GCGR), a class B seven-transmembrane G protein-coupled receptor that couples to the stimulatory heterotrimeric G protein and provokes PKA-dependent signaling cascades vital to hepatic glucose metabolism and islet insulin secretion. The GCGR expressed at the plasma membrane is constitutively ubiquitinated and upon agonist-activation, internalized GCGRs are deubiquitinated at early endosomes and recycled via Rab4-containing vesicles. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Biotinylated Human GCGR/Glucagon receptor Protein, Ultra Low Endotoxin (GCR-HM40RB-UL) Specifications/Details
Protein |
GCGR/Glucagon receptor |
Synonyms |
Glucagon receptor; GL-R; GCGR; Glucagon R |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Ala26-Lys136 |
Molecular Weight |
The protein has a predicted MW of 15.96 kDa. Due to glycosylation, the protein migrates to 30-50 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human GCGR, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-GCGR Antibody, hFc Tag with the EC50 of 11.5ng/ml determined by ELISA. |
Product Tag |
C-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Biotinylated Human GCGR, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-GCGR Antibody, hFc Tag with the EC50 of 11.5ng/ml determined by ELISA. |
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Biotinylated Human GCGR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human GCGR is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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