Biotinylated Human GDF15 (H202D) Protein (Primary Amine Labeling) (GDF-HM216B)
  • Express System: HEK293
  • Product Tag: N-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human GFRAL, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GDF15 (H202D), hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ala197-Ile308 (H202D)
Catalog #GDF-HM216B
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Product Description
 Recombinant Biotinylated Human GDF15 (H202D) Protein (Primary Amine Labeling) is expressed from HEK293 with hFc tag at the N-terminus.It contains Ala197-Ile308 (H202D).
Product Background
 Growth and differentiation factor 15 (GDF15) is an inflammation-associated hormone with poorly defined biology. Here, we investigated the role of GDF15 in bacterial and viral infections. Inflammation induced GDF15, and that GDF15 was necessary for surviving both bacterial and viral infections, as well as sepsis. The protective effects of GDF15 were largely independent of pathogen control or the magnitude of inflammatory response, suggesting a role in disease tolerance.
Product Category
 Recombinant Protein
Target
 GDF15 (H202D)
Synonyms
 GDF-15; MIC-1; NAG-1; PDF; PLAB; PTGFB; GDF15; MIC1; RG-1; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta; Placental TGF-β; PTGF-β; PTGFBPTGF-β
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Ala197-Ile308 (H202D)
Molecular Weight
 The protein has a predicted MW of 37.88 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human GFRAL, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GDF15 (H202D), hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. See testing image for detail.
Product Tag
 N-hFc
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human GFRAL, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GDF15 (H202D), hFc Tag with the EC50 of 4.4ng/ml determined by ELISA.
Biotinylated Human GDF15 (H202D) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human GDF15 (H202D) is greater than 95% as determined by SEC-HPLC.

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