Biotinylated Human GDF15 Protein (GDF-HE415B) Summary
Product Description |
Recombinant Biotinylated Human GDF15 Protein is expressed from E.coli with His tag and Avi tag at the N-Terminus.; It contains Ala197-Ile308. |
Product Background |
Growth Differentiation Factor 15 (GDF15), also known as NSAID activated gene-1 (NAG-1), is associated with a large number of biological processes and diseases, including cancer and obesity. GDF15 is synthesized as pro-GDF15, is dimerized, and is cleaved and secreted into the circulation as a mature dimer GDF15. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Biotinylated Human GDF15 Protein (GDF-HE415B) Specifications/Details
Protein |
GDF15 |
Synonyms |
GDF-15; MIC-1; NAG-1; PDF; PLAB; PTGFB; GDF15; MIC1; RG-1; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta; Placental TGF-β; PTGF-β; PTGFBPTGF-β |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Ala197-Ile308 |
Molecular Weight |
The protein has a predicted MW of 15.19 kDa same as Bis-Tris PAGE result. |
Activity |
Immobilized Human GFRAL, hFc Tag at 5 ug/ml (100 ul/Well) on the plate. Dose response curve for Biotinylated Human GDF15, His Tag with the EC50 of 37.9 ng/ml determined by ELISA (QC Test). |
Product Tag |
N-His-Avi |
Expression System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in 50 mM HAc (pH 2.9). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in 50mM HAc (pH 2.9). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Immobilized Human GFRAL, hFc Tag at 5 ug/ml (100 ul/Well) on the plate. Dose response curve for Biotinylated Human GDF15, His Tag with the EC50 of 37.9 ng/ml determined by ELISA (QC Test). |
|
|
Biotinylated Human GDF15 on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 95%. |
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