Biotinylated Human GDF15 Protein (GDF-HE415B)

  • Express System: E.coli
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Biotinylated: yes
  • Activity: Immobilized Human GFRAL, hFc Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Biotinylated Human GDF15, His Tag with the EC50 of 24 ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ala197-Ile308

Catalog: GDF-HE415B

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Product Description Recombinant Biotinylated Human GDF15 Protein is expressed from E.coli with His tag and Avi tag at the N-Terminus.; It contains Ala197-Ile308.

Product Background

 Growth Differentiation Factor 15 (GDF15), also known as NSAID activated gene-1 (NAG-1), is associated with a large number of biological processes and diseases, including cancer and obesity. GDF15 is synthesized as pro-GDF15, is dimerized, and is cleaved and secreted into the circulation as a mature dimer GDF15.

Product Category

 Recombinant Protein

Target

 Biotinylated GDF15
Synonyms GDF-15; MIC-1; NAG-1; PDF; PLAB; PTGFB; GDF15; MIC1; RG-1; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta
Accession

Species

 Human

Amino Acid Range

 Ala197-Ile308
Molecular Weight The protein has a predicted MW of 15.19 kDa same as Tris-Bis PAGE result.

Activity

 Immobilized Human GFRAL, hFc Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Biotinylated Human GDF15, His Tag with the EC50 of 24 ng/ml determined by ELISA. See testing image for detail.

Product Tag

 N-His-Avi

Express System

 E.coli
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1 EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in 50 mM HAc (pH 2.9). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 50mM HAc (pH 2.9).
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human GFRAL, hFc Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Biotinylated Human GDF15, His Tag with the EC50 of 24 ng/ml determined by ELISA.
Biotinylated Human GDF15 on Tris-Bis PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%.

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