Biotinylated Human GDF15 Protein (Primary Amine Labeling) (GDF-HM215B)

  • Express System: Expi293
  • Product Tag: N-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Human GFRAL, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human GDF15, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ala197-Ile308

Catalog: GDF-HM215B

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Product Description Recombinant Biotinylated Human GDF15 Protein (Primary Amine Labeling) is expressed from Expi293 with hFc tag at the N-terminal.It contains Ala197-Ile308.
Product Category Recombinant Protein
Target GDF15
Synonyms GDF-15; MIC-1; NAG-1; PDF; PLAB; PTGFB; GDF15; MIC1; RG-1; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta
Gene/Protein ID Q99988
Species Human
Exact Sequence Ala197-Ile308
Molecular Weight The protein has a predicted MW of 37.9 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human GFRAL, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human GDF15, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA. See testing image for detail.
Product Tag N-hFc
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human GFRAL, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human GDF15, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA.
The purity of Biotinylated Human GDF15 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human GDF15 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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