Biotinylated Human GDF15 Protein (Primary Amine Labeling) (GDF-HM215B)

  • Express System: Expi293
  • Product Tag: N-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Biotinylated: yes
  • Activity: Immobilized Human GFRAL, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human GDF15, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ala197-Ile308

Catalog: GDF-HM215B

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Product Description Recombinant Biotinylated Human GDF15 Protein (Primary Amine Labeling) is expressed from Expi293 with hFc tag at the N-terminal.It contains Ala197-Ile308.
Product Category Recombinant Protein
Target GDF15
Synonyms GDF-15; MIC-1; NAG-1; PDF; PLAB; PTGFB; GDF15; MIC1; RG-1; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta
Gene/Protein ID Q99988
Species Human
Exact Sequence Ala197-Ile308
Molecular Weight The protein has a predicted MW of 37.9 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human GFRAL, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human GDF15, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA. See testing image for detail.
Product Tag N-hFc
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human GFRAL, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human GDF15, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA.
Biotinylated Human GDF15 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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