- Express System: HEK293
- Product Tag: C-His-Avi
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: Immobilized Biotinylated Human GIPR, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-GIPR Antibody, hFc Tag with the EC50 of 12.2ng/ml determined by ELISA.
- Amino Acid Range: Gly26-Gln138
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Biotinylated Human GIPR Protein (GIP-HM40RB) Summary
Product Description |
Recombinant Biotinylated Human GIPR Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Gly26-Gln138. |
Product Background |
The gastric inhibitory polypeptide receptor (GIPR), a G protein-coupled receptor (GPCR) that regulates glucose metabolism and insulin secretion, is a target for the development of therapeutic agents to address type 2 diabetes and obesity. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Biotinylated Human GIPR Protein (GIP-HM40RB) Specifications/Details
Protein |
GIPR |
Synonyms |
GIP-R; GIPR; PGQTL2 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Gly26-Gln138 |
Molecular Weight |
The protein has a predicted MW of 15.88 kDa. Due to glycosylation, the protein migrates to 28-38 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human GIPR, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-GIPR Antibody, hFc Tag with the EC50 of 12.2ng/ml determined by ELISA. |
Product Tag |
C-His-Avi |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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