Name: Biotinylated Human GITR Ligand/TNFSF18 Trimer Protein (Primary Amine Labeling) (FSF-HM418B)
Brand: KactusBio
SKU: FSF-HM418B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human GITR Ligand/TNFSF18 Trimer Protein (Primary Amine Labeling) (FSF-HM418B)

Immobilized Human GITR, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GITR Ligand Trimer, His Tag with the EC50 of 11.4ng/ml determined by ELISA.

Biotinylated Human GITR Ligand Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human GITR Ligand Trimer is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: N-His-Flag
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human GITR, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GITR Ligand Trimer, His Tag with the EC50 of 11.4ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Gln50-Ser177

Catalog #FSF-HM418B

Size

100UG

500UG

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Biotinylated Human GITR Ligand/TNFSF18 Trimer Protein (Primary Amine Labeling) (FSF-HM418B) Summary

Product Description	Recombinant Biotinylated Human GITR Ligand/TNFSF18 Trimer Protein (Primary Amine Labeling) is expressed from HEK293 with His tag and flag tag at the N-Terminus.It contains Gln50-Ser177.
Product Background	Glucocorticoid-induced TNFR-related protein (TNFRSF18, GITR, CD357), expressed by T cells, and its ligand (TNFSF18, GITRL), expressed by myeloid populations, provide co-stimulatory signals that boost T cell activity. Due to the important role that GITR plays in regulating immune functions, agonistic stimulation of GITR is a promising therapeutic concept.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Biotinylated Human GITR Ligand/TNFSF18 Trimer Protein (Primary Amine Labeling) (FSF-HM418B) Specifications/Details

Protein	GITR Ligand/TNFSF18
Synonyms	TNFSF18; AITRL; TL6; GITRL; GITR Ligand
Accession	Q9UNG2
Species	Human
Biotinylated	yes
Amino Acid Range	Gln50-Ser177
Molecular Weight	The protein has a predicted MW of 47.5 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human GITR, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GITR Ligand Trimer, His Tag with the EC50 of 11.4ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-His-Flag
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Please refer to the Certificate of Analysis for detailed instructions.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human GITR, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GITR Ligand Trimer, His Tag with the EC50 of 11.4ng/ml determined by ELISA.
	Biotinylated Human GITR Ligand Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human GITR Ligand Trimer is greater than 95% as determined by SEC-HPLC.