Biotinylated Human GITR Ligand/TNFSF18 Trimer Protein (Primary Amine Labeling) (FSF-HM418B)
Catalog: FSF-HM418B
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Product Description | Recombinant Biotinylated Human GITR Ligand/TNFSF18 Trimer Protein (Primary Amine Labeling) is expressed from HEK293 with His tag and flag tag at the N-Terminus.It contains Gln50-Ser177. |
Product Background |
Glucocorticoid-induced TNFR-related protein (TNFRSF18, GITR, CD357), expressed by T cells, and its ligand (TNFSF18, GITRL), expressed by myeloid populations, provide co-stimulatory signals that boost T cell activity. Due to the important role that GITR plays in regulating immune functions, agonistic stimulation of GITR is a promising therapeutic concept. |
Product Category |
Recombinant Protein |
Target |
GITR Ligand/TNFSF18 Trimer |
Synonyms | TNFSF18; AITRL; TL6; GITRL; GITR Ligand |
Accession | |
Species |
Human |
Amino Acid Range |
Gln50-Ser177 |
Molecular Weight | The protein has a predicted MW of 47.5 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Human GITR, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GITR Ligand Trimer, His Tag with the EC50 of 11.4ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
N-His-Flag |
Express System |
HEK293 |
Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Endotoxin | Less than 1EU per ug by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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