Biotinylated Human GITR Ligand/TNFSF18 Trimer Protein (Primary Amine Labeling) (FSF-HM418B)

  • Express System: HEK293
  • Product Tag: N-His-Flag
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Human GITR, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GITR Ligand Trimer, His Tag with the EC50 of 11.4ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln50-Ser177

Catalog: FSF-HM418B

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Product Description Recombinant Biotinylated Human GITR Ligand/TNFSF18 Trimer Protein (Primary Amine Labeling) is expressed from HEK293 with His tag and flag tag at the N-Terminus.It contains Gln50-Ser177.

Product Background

Glucocorticoid-induced TNFR-related protein (TNFRSF18, GITR, CD357), expressed by T cells, and its ligand (TNFSF18, GITRL), expressed by myeloid populations, provide co-stimulatory signals that boost T cell activity. Due to the important role that GITR plays in regulating immune functions, agonistic stimulation of GITR is a promising therapeutic concept.

Product Category

Recombinant Protein

Target

GITR Ligand/TNFSF18 Trimer
Synonyms TNFSF18; AITRL; TL6; GITRL; GITR Ligand

Gene/Protein ID

Q9UNG2

Species

Human

Exact Sequence

Gln50-Ser177
Molecular Weight The protein has a predicted MW of 47.5 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human GITR, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GITR Ligand Trimer, His Tag with the EC50 of 11.4ng/ml determined by ELISA. See testing image for detail.

Product Tag

N-His-Flag

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human GITR, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GITR Ligand Trimer, His Tag with the EC50 of 11.4ng/ml determined by ELISA.
The purity of Biotinylated Human GITR Ligand Trimer is greater than 95% as determined by SEC-HPLC.
Biotinylated Human GITR Ligand Trimer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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