Biotinylated Human GITR Ligand/TNFSF18 Trimer Protein (Primary Amine Labeling) (FSF-HM418B)
Biotinylated Human GITR Ligand/TNFSF18 Trimer Protein (Primary Amine Labeling) (FSF-HM418B)
Biotinylated Human GITR Ligand/TNFSF18 Trimer Protein (Primary Amine Labeling) (FSF-HM418B)
Biotinylated Human GITR Ligand/TNFSF18 Trimer Protein (Primary Amine Labeling) (FSF-HM418B)
  • Express System: HEK293
  • Product Tag: N-His-Flag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human GITR, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GITR Ligand Trimer, His Tag with the EC50 of 11.4ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gln50-Ser177
Catalog #FSF-HM418B
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Product Description
 Recombinant Biotinylated Human GITR Ligand/TNFSF18 Trimer Protein (Primary Amine Labeling) is expressed from HEK293 with His tag and flag tag at the N-Terminus.It contains Gln50-Ser177.
Product Background
 Glucocorticoid-induced TNFR-related protein (TNFRSF18, GITR, CD357), expressed by T cells, and its ligand (TNFSF18, GITRL), expressed by myeloid populations, provide co-stimulatory signals that boost T cell activity. Due to the important role that GITR plays in regulating immune functions, agonistic stimulation of GITR is a promising therapeutic concept.
Product Category
 Recombinant Protein
Target
 GITR Ligand/TNFSF18 Trimer
Synonyms
 TNFSF18; AITRL; TL6; GITRL; GITR Ligand
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Gln50-Ser177
Molecular Weight
 The protein has a predicted MW of 47.5 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human GITR, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GITR Ligand Trimer, His Tag with the EC50 of 11.4ng/ml determined by ELISA. See testing image for detail.
Product Tag
 N-His-Flag
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human GITR, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GITR Ligand Trimer, His Tag with the EC50 of 11.4ng/ml determined by ELISA.
Biotinylated Human GITR Ligand Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human GITR Ligand Trimer is greater than 95% as determined by SEC-HPLC.

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