Biotinylated Human GITR Ligand/TNFSF18 Trimer Protein (Primary Amine Labeling) (FSF-HM418B)

  • Express System: Expi293
  • Product Tag: N-His-Flag
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Human GITR, His Tag at 1μg/ml (100μl/Well). Dose response curve for Biotinylated Human GITR Ligand Trimer, His Tag with the EC50 of 0.2μg/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln50-Ser177

Catalog: FSF-HM418B

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Product Description Recombinant Biotinylated Human GITR Ligand/TNFSF18 Trimer Protein (Primary Amine Labeling) is expressed from Expi293 with His tag and flag tag at the N-terminal.It contains Gln50-Ser177.
Product Category Recombinant Protein
Target GITR Ligand/TNFSF18 Trimer
Synonyms TNFSF18; AITRL; TL6; GITRL; GITR Ligand
Gene/Protein ID Q9UNG2
Species Human
Exact Sequence Gln50-Ser177
Molecular Weight The protein has a predicted MW of 47.5 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human GITR, His Tag at 1μg/ml (100μl/Well). Dose response curve for Biotinylated Human GITR Ligand Trimer, His Tag with the EC50 of 0.2μg/ml determined by ELISA. See testing image for detail.
Product Tag N-His-Flag
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human GITR, His Tag at 1μg/ml (100μl/Well). Dose response curve for Biotinylated Human GITR Ligand Trimer, His Tag with the EC50 of 0.2μg/ml determined by ELISA.
The purity of Biotinylated Human GITR Ligand Trimer is greater than 95% as determined by SEC-HPLC.
Biotinylated Human GITR Ligand Trimer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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