Biotinylated Human GITR/TNFRSF18 Protein (GTR-HM401B)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Human GITR Ligand Trimer, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human GITR, His Tag with the EC50 of 0.14μg/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln26-Glu161

Catalog: GTR-HM401B

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Product Description Recombinant Biotinylated Human GITR/TNFRSF18 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Gln26-Glu161.
Product Category Recombinant Protein
Target GITR/TNFRSF18
Synonyms CD357; GITR; GITR-D; TNFRSF18; AITR
Gene/Protein ID Q9Y5U5-1
Species Human
Exact Sequence Gln26-Glu161
Molecular Weight The protein has a predicted MW of 17.2 kDa. Due to glycosylation, the protein migrates to 26-28 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human GITR Ligand Trimer, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human GITR, His Tag with the EC50 of 0.14μg/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human GITR Ligand Trimer, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human GITR, His Tag with the EC50 of 0.14μg/ml determined by ELISA.
The purity of Biotinylated Human GITR is greater than 95% as determined by SEC-HPLC.
Biotinylated Human GITR on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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