Product Description |
Recombinant Biotinylated Human GITR/TNFRSF18 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln26-Glu161. |
Product Background |
GITR (glucocorticoid-induced tumor necrosis factor receptor), also known as AITR and TNFRSF18, is a 40 kDa transmembrane glycoprotein that functions in immune regulation.GIRT is a receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway. |
Product Category |
Recombinant Protein |
Target |
GITR/TNFRSF18 |
Synonyms |
CD357; GITR; GITR-D; TNFRSF18; AITR |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Gln26-Glu161 |
Molecular Weight |
The protein has a predicted MW of 17.2 kDa. Due to glycosylation, the protein migrates to 26-28 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human GITR Ligand Trimer, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GITR, His Tag with the EC50 of 77.9ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human GITR Ligand Trimer, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GITR, His Tag with the EC50 of 77.9ng/ml determined by ELISA. |
|
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Biotinylated Human GITR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human GITR is greater than 95% as determined by SEC-HPLC. |
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