Name: Biotinylated Human GM-CSF R alpha Protein (CSF-HM42RB)
Brand: KactusBio
SKU: CSF-HM42RB-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Human GM-CSF R alpha Protein (CSF-HM42RB)

Biotinylated Human GM-CSF R alpha, His-Avi Tag captured on CM5 Chip via Anti-His Antibody can bind Human GM-CSF, No Tag with an affinity constant of 1.68 nM as determined in SPR assay (Biacore T200).

Immobilized Anti-GM-CSF R alpha Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GM-CSF R alpha, His Tag with the EC50 of 11.6ng/ml determined by ELISA (QC Test).

Biotinylated Human GM-CSF R alpha on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.

The purity of Biotinylated Human GM-CSF R alpha is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Anti-GM-CSF R alpha Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GM-CSF R alpha, His Tag with the EC50 of 11.6ng/ml determined by ELISA (QC Test). The affinity constant of 1.68 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Glu23-Gly320

Catalog #CSF-HM42RB

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human GM-CSF R alpha Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu23-Gly320.
Product Background	In the first trimester, the GM-CSF-R was found to be present on villous cytotrophoblast and all populations of extravillous trophoblast. Expression by villous syncytiotrophoblast was weak or absent, but this increased markedly by term. GM-CSF-R were also expressed by fetal Hofbauer cells within the mesenchyme of the chorionic villi and by uterine glandular epithelium and decidual macrophages within maternal decidua.
Product Category	Recombinant Protein / Antibody discovery

Protein	GM-CSF R alpha
Synonyms	GM-CSF-R-alpha; GMCSFR-alpha; GMR-alpha; CSF2R; CSF2RY; CSF2RA; CDw116; CD116; CSF2RAX; CSF2RAY; CSF2RX; GMCSFR; GMR; SMDP4
Accession	P15509-1
Species	Human
Biotinylated	yes
Amino Acid Range	Glu23-Gly320
Molecular Weight	The protein has a predicted MW of 37.7 kDa. Due to glycosylation, the protein migrates to 68-75 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Anti-GM-CSF R alpha Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GM-CSF R alpha, His Tag with the EC50 of 11.6ng/ml determined by ELISA (QC Test). The affinity constant of 1.68 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Biotinylated Human GM-CSF R alpha, His-Avi Tag captured on CM5 Chip via Anti-His Antibody can bind Human GM-CSF, No Tag with an affinity constant of 1.68 nM as determined in SPR assay (Biacore T200).
	Immobilized Anti-GM-CSF R alpha Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GM-CSF R alpha, His Tag with the EC50 of 11.6ng/ml determined by ELISA (QC Test).
	Biotinylated Human GM-CSF R alpha on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
	The purity of Biotinylated Human GM-CSF R alpha is greater than 95% as determined by SEC-HPLC.