Biotinylated Human GM-CSF R alpha Protein (CSF-HM42RB)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Anti-GM-CSF R alpha Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GM-CSF R alpha, His Tag with the EC50 of 11.6ng/ml determined by ELISA (QC Test). The affinity constant of 1.68 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Glu23-Gly320
Catalog #CSF-HM42RB
Regular price $577.50
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Product Description

Recombinant Biotinylated Human GM-CSF R alpha Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu23-Gly320.

Product Background

In the first trimester, the GM-CSF-R was found to be present on villous cytotrophoblast and all populations of extravillous trophoblast. Expression by villous syncytiotrophoblast was weak or absent, but this increased markedly by term. GM-CSF-R were also expressed by fetal Hofbauer cells within the mesenchyme of the chorionic villi and by uterine glandular epithelium and decidual macrophages within maternal decidua.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Protein

GM-CSF R alpha

Synonyms

GM-CSF-R-alpha; GMCSFR-alpha; GMR-alpha; CSF2R; CSF2RY; CSF2RA; CDw116; CD116; CSF2RAX; CSF2RAY; CSF2RX; GMCSFR; GMR; SMDP4

Accession

Species

Human

Biotinylated

yes

Amino Acid Range

Glu23-Gly320

Molecular Weight

The protein has a predicted MW of 37.7 kDa. Due to glycosylation, the protein migrates to 68-75 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Anti-GM-CSF R alpha Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GM-CSF R alpha, His Tag with the EC50 of 11.6ng/ml determined by ELISA (QC Test). The affinity constant of 1.68 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biotinylated Human GM-CSF R alpha, His-Avi Tag captured on CM5 Chip via Anti-His Antibody can bind Human GM-CSF, No Tag with an affinity constant of 1.68 nM as determined in SPR assay (Biacore T200). Biotinylated Human GM-CSF R alpha, His-Avi Tag captured on CM5 Chip via Anti-His Antibody can bind Human GM-CSF, No Tag with an affinity constant of 1.68 nM as determined in SPR assay (Biacore T200).
Immobilized Anti-GM-CSF R alpha Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GM-CSF R alpha, His Tag with the EC50 of 11.6ng/ml determined by ELISA (QC Test). Immobilized Anti-GM-CSF R alpha Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GM-CSF R alpha, His Tag with the EC50 of 11.6ng/ml determined by ELISA (QC Test).
Biotinylated Human GM-CSF R alpha on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. Biotinylated Human GM-CSF R alpha on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Biotinylated Human GM-CSF R alpha is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human GM-CSF R alpha is greater than 95% as determined by SEC-HPLC.

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