Biotinylated Human GM-CSF R alpha Protein (CSF-HM42RB)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Anti-GM-CSF R alpha Antibody, hFc Tag at 2_g/ml (100_l/well) on the plate. Dose response curve for Biotinylated Human GM-CSF R alpha, His Tag with the EC50 of 11.3ng/ml determined by ELISA. The affinity constant of 1.68 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Glu23-Gly320

Catalog: CSF-HM42RB

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Product Description Recombinant Biotinylated Human GM-CSF R alpha Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Glu23-Gly320.
Product Category Recombinant Protein
Target GM-CSF R alpha
Synonyms GM-CSF-R-alpha; GMCSFR-alpha; GMR-alpha; CSF2R; CSF2RY; CSF2RA; CDw116; CD116; CSF2RAX; CSF2RAY; CSF2RX; GMCSFR; GMR; SMDP4
Gene/Protein ID P15509-1
Species Human
Exact Sequence Glu23-Gly320
Molecular Weight The protein has a predicted MW of 37.7 kDa. Due to glycosylation, the protein migrates to 68-75 kDa based on Tris-Bis PAGE result.
Activity Immobilized Anti-GM-CSF R alpha Antibody, hFc Tag at 2_g/ml (100_l/well) on the plate. Dose response curve for Biotinylated Human GM-CSF R alpha, His Tag with the EC50 of 11.3ng/ml determined by ELISA. The affinity constant of 1.68 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per _g by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22_m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 _g/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80¡C for 12 months, 4¡C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Biotinylated Human GM-CSF R alpha, His-Avi Tag captured on CM5 Chip via Anti-His Antibody can bind Human GM-CSF, No Tag with an affinity constant of 1.68 nM as determined in SPR assay (Biacore T200).
Immobilized Anti-GM-CSF R alpha Antibody, hFc Tag at 2_g/ml (100_l/well) on the plate. Dose response curve for Biotinylated Human GM-CSF R alpha, His Tag with the EC50 of 11.3ng/ml determined by ELISA.
The purity of Biotinylated Human GM-CSF R alpha is greater than 95% as determined by SEC-HPLC.
Biotinylated Human GM-CSF R alpha on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

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