Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer Protein (MHC-HM402B)
Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer Protein (MHC-HM402B)
Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer Protein (MHC-HM402B)
Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer Protein (MHC-HM402B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and YLEPGPVTA peptide
Catalog #MHC-HM402B
Regular price $310.00
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Product Description
Recombinant Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and YLEPGPVTA peptide.
Product Background
Glycoprotein 100, gp100 or Melanocyte protein PMEL is 661 amino acids long and is a type I transmembrane glycoprotein enriched in melanosomes, which are the melanin-producing organelles in melanocytes. This protein is involved in melanosome maturation. The Human HLA-A*0201 gp100 (YLEPGPVTA) complex Protein is a complex of HLA-A*0201 of -the MHC Class I and YLEPGPVTA peptide of -the gp100.
Product Category
Recombinant Protein
Target
HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer
Synonyms
HLA0201; MHC I; gp100; Glycoprotein 100; ME20-M; P100
MHC Form
Monomer
Allele
HLA-A*02:01
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and YLEPGPVTA peptide
Molecular Weight
The protein has a predicted MW of 50.4 kDa. Due to glycosylation, the protein migrates to 52-62 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA (QC Test).
Immobilized Tebentafusp, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer, His Tag with the EC50 of 11.8ng/ml determined by ELISA.
Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer was greater than 95% as determined by SEC-HPLC.

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