Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer Protein (MHC-HM402B)
Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer Protein (MHC-HM402B)
Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer Protein (MHC-HM402B)
Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer Protein (MHC-HM402B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and YLEPGPVTA peptide
Catalog #MHC-HM402B
Regular price $341.00
/
Size
image

Order now, ships by:

Product Description

Recombinant Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and YLEPGPVTA peptide.

Product Background

Glycoprotein 100, gp100 or Melanocyte protein PMEL is 661 amino acids long and is a type I transmembrane glycoprotein enriched in melanosomes, which are the melanin-producing organelles in melanocytes. This protein is involved in melanosome maturation. The Human HLA-A*0201 gp100 (YLEPGPVTA) complex Protein is a complex of HLA-A*0201 of -the MHC Class I and YLEPGPVTA peptide of -the gp100.

Product Category

Recombinant Protein / Antibody discovery / MHC / Peptide-MHCs

Protein

HLA-A*02:01&B2M&GP100 (YLEPGPVTA)

Synonyms

HLA0201; MHC I; gp100; Glycoprotein 100; ME20-M; P100

MHC Form

Monomer

Allele

HLA-A*02:01

Accession

Species

Human

Biotinylated

yes

Amino Acid Range

Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and YLEPGPVTA peptide

Molecular Weight

The protein has a predicted MW of 50.4 kDa. Due to glycosylation, the protein migrates to 52-62 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 24 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA (QC Test). Immobilized Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA (QC Test).
Immobilized Tebentafusp, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer, His-Avi Tag with the EC50 of 21.7ng/ml determined by ELISA. Immobilized Tebentafusp, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer, His-Avi Tag with the EC50 of 21.7ng/ml determined by ELISA.
Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer was greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer was greater than 95% as determined by SEC-HPLC.

Have more questions?

Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.

Contact Us

support@kactusbio.us

Recently viewed