Biotinylated Human GP100 (HLA-A*02:01) Protein (MHC-HM402B)
Catalog: MHC-HM402B
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Product Description | Recombinant Biotinylated Human GP100 (HLA-A*02:01) Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and YLEPGPVTA peptide. |
Product Category | Recombinant Protein |
Target | GP100 (HLA-A*02:01) |
Synonyms | HLA0201; MHC I; gp100; Glycoprotein 100; ME20-M; P100 |
Gene/Protein ID | A0A140T913(HLA-A*02:01)&P61769(B2M)&YLEPGPVTA |
Species | Human |
Exact Sequence | Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and YLEPGPVTA peptide |
Molecular Weight | The protein has a predicted MW of 50.4 kDa. Due to glycosylation, the protein migrates to 52-62 kDa based on Tris-Bis PAGE result. |
Activity | Immobilized Biotinylated Human GP100 (HLA-A*02:01) , His Tag at 2μg/ml (100μl/Well). Dose response curve for Anti-GP100 (HLA-A*02:01) Ab., hFc tag with the EC50 of 0.3μg/ml determined by ELISA. See testing image for detail. |
Product Tag | C-His-Avi |
Express System | Expi293 |
Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Endotoxin | Less than 1EU per μg by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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