Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer Protein (MHC-HM402B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Anti-HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Antibody, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer, His Tag with the EC50 of 0.88ug/ml determined by ELISA (QC Test). See testing image for detail.
  • Exact Sequence: Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and YLEPGPVTA peptide

Catalog: MHC-HM402B

$310.00
Size:

Order now get it on

Product Description Recombinant Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and YLEPGPVTA peptide.

Product Background

Glycoprotein 100, gp100 or Melanocyte protein PMEL is 661 amino acids long and is a type I transmembrane glycoprotein enriched in melanosomes, which are the melanin-producing organelles in melanocytes. This protein is involved in melanosome maturation. The Human HLA-A*0201 gp100 (YLEPGPVTA) complex Protein is a complex of HLA-A*0201 of -the MHC Class I and YLEPGPVTA peptide of -the gp100.

Product Category

Recombinant Protein

Target

HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer
Synonyms HLA0201; MHC I; gp100; Glycoprotein 100; ME20-M; P100
MHC Form Monomer
Allele HLA-A*02:01

Gene/Protein ID

A0A140T913(HLA-A*02:01)&P61769(B2M)&YLEPGPVTA

Species

Human

Exact Sequence

Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and YLEPGPVTA peptide
Molecular Weight The protein has a predicted MW of 50.4 kDa. Due to glycosylation, the protein migrates to 52-62 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Anti-HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Antibody, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer, His Tag with the EC50 of 0.88ug/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Antibody, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer, His Tag with the EC50 of 0.88ug/ml determined by ELISA (QC Test).
Immobilized Tebentafusp, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer, His Tag with the EC50 of 11.8ng/ml determined by ELISA.
The purity of Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer was greater than 95% as determined by SEC-HPLC.
Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

You may also be interested in