Product Description |
Recombinant Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and YLEPGPVTA peptide. |
Product Background |
Glycoprotein 100, gp100 or Melanocyte protein PMEL is 661 amino acids long and is a type I transmembrane glycoprotein enriched in melanosomes, which are the melanin-producing organelles in melanocytes. This protein is involved in melanosome maturation. The Human HLA-A*0201 gp100 (YLEPGPVTA) complex Protein is a complex of HLA-A*0201 of -the MHC Class I and YLEPGPVTA peptide of -the gp100. |
Product Category |
Recombinant Protein |
Target |
HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer |
Synonyms |
HLA0201; MHC I; gp100; Glycoprotein 100; ME20-M; P100 |
MHC Form |
Monomer |
Allele |
HLA-A*02:01 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and YLEPGPVTA peptide |
Molecular Weight |
The protein has a predicted MW of 50.4 kDa. Due to glycosylation, the protein migrates to 52-62 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA (QC Test). |
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Immobilized Tebentafusp, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer, His Tag with the EC50 of 11.8ng/ml determined by ELISA. |
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Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer was greater than 95% as determined by SEC-HPLC. |
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