Biotinylated Human HLA-A*24:02&B2M&GP100 Intron 4 (VYFFLPDHL) Monomer Protein (MHC-HM433B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Exact Sequence: Gly25-Thr305(HLA-A*24:02), Ile21-Met119(B2M)and VYFFLPDHL peptide

Catalog: MHC-HM433B

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Product Description Recombinant Biotinylated Human HLA-A*24:02&B2M&GP100 Intron 4 (VYFFLPDHL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*24:02), Ile21-Met119(B2M) and VYFFLPDHL peptide.

Product Background

 Glycoprotein 100, gp100 or Melanocyte protein PMEL is 661 amino acids long and is a type I transmembrane glycoprotein enriched in melanosomes, which are the melanin-producing organelles in melanocytes. This protein is involved in melanosome maturation. The Human HLA-A*0201 gp100 (YLEPGPVTA) complex Protein is a complex of HLA-A*0201 of- the MHC Class I, B2M and YLEPGPVTA peptide of- the gp100.

Product Category

 Recombinant Protein

Target

 HLA-A*24:02&B2M&GP100 Intron 4 (VYFFLPDHL) Monomer
MHC Form Monomer
Allele HLA-A*24:02

Gene/Protein ID

 AAA59600.1(HLA-A*24:02)&P61769(B2M)&VYFFLPDHL

Species

 Human

Exact Sequence

 Gly25-Thr305(HLA-A*24:02), Ile21-Met119(B2M)and VYFFLPDHL peptide
Molecular Weight The protein has a predicted MW of 50.40 kDa. Due to glycosylation, the protein migrates to 55-63 kDa based on Tris-Bis PAGE result.

Product Tag

 C-His-Avi

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
The purity of Biotinylated Human HLA-A*24:02&B2M&GP100 Intron 4 (VYFFLPDHL) Monomer is greater than 95% as determined by SEC-HPLC.
Biotinylated Human HLA-A*24:02&B2M&GP100 Intron 4 (VYFFLPDHL) Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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