Biotinylated Human GPA33/A33 Protein (GPA-HM433B)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Anti-GPA33 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human GPA33, His Tag with the EC50 of 11.0ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ile22-Val235

Catalog: GPA-HM433B

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Product Description Recombinant Biotinylated Human GPA33/A33 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Ile22-Val235.
Product Category Recombinant Protein
Target GPA33/A33
Synonyms Glycoprotein A33; GPA33; A33; MGC129986; MGC129987
Gene/Protein ID Q99795-1
Species Human
Exact Sequence Ile22-Val235
Molecular Weight The protein has a predicted MW of 26.5 kDa. Due to glycosylation, the protein migrates to 28-40 kDa based on Tris-Bis PAGE result.
Activity Immobilized Anti-GPA33 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human GPA33, His Tag with the EC50 of 11.0ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-GPA33 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human GPA33, His Tag with the EC50 of 11.0ng/ml determined by ELISA.
The purity of Biotinylated Human GPA33 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human GPA33 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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