Biotinylated Human GPA33/A33 Protein (GPA-HM433B)
Biotinylated Human GPA33/A33 Protein (GPA-HM433B)
Biotinylated Human GPA33/A33 Protein (GPA-HM433B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human GPA33, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-GP33 Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Ile22-Val235
Catalog #GPA-HM433B
Regular price $577.50
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Product Description

Recombinant Biotinylated Human GPA33/A33 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ile22-Val235.

Product Background

Glycoprotein A33 (GPA33) is also known as Cell surface A33 antigen, is a single-pass type I membrane protein which is expressed in normal gastrointestinal epithelium and in 95% of colon cancers. GPA33 The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 1 Ig-like C2-type (immunoglobulin-like) domain and 1 Ig-like V-type (immunoglobulin-like) domain characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily, which contains. GPA33 may play a role in cell-cell recognition and signaling.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Protein

GPA33/A33

Synonyms

Glycoprotein A33; GPA33; A33; MGC129986; MGC129987

Accession

Species

Human

Biotinylated

yes

Amino Acid Range

Ile22-Val235

Molecular Weight

The protein has a predicted MW of 26.5 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Biotinylated Human GPA33, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-GP33 Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human GPA33, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-GP33 Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA (QC Test). Immobilized Biotinylated Human GPA33, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-GP33 Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA (QC Test).
Biotinylated Human GPA33 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human GPA33 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human GPA33 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human GPA33 is greater than 95% as determined by SEC-HPLC.

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