Biotinylated Human GPRC5D Nanodisc (GPR-HM15NB)
  • Express System: HEK293
  • Product Tag: C-His
  • Activity: Immobilized Biotinylated Human GPRC5D Nanodisc, His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate(5ug/ml). Dose response curve for Anti-GPRC5D Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA (QC Test).
  • Amino Acid Range: Met1-Val345
Catalog #GPR-HM15NB
Regular price $2,165.90
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Biotinylated Human GPRC5D Nanodisc (GPR-HM15NB) Summary

Product Description

Recombinant Biotinylated Human GPRC5D Nanodisc is expressed from HEK293 with His tag at the C-terminus.It contains Met1-Val345.

Product Background

G protein–coupled receptor, family C, group 5, member D (GPRC5D) is an orphan receptor that is primarily expressed in plasma cells and hard keratinized tissues, with low expression in normal human tissues. GPRC5D expression is enriched in malignant plasma cells and is associated with markers of high-risk myeloma.

Product Category

Recombinant Protein / Antibody discovery / Multiple transmembrane proteins / Nanodisc

Biotinylated Human GPRC5D Nanodisc (GPR-HM15NB) Specifications/Details

Protein
GPRC5D
Synonyms
GPRC5D; MGC129713; MGC129714; orphan G-protein coupled receptor
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Met1-Val345
Molecular Weight
The protein has a predicted MW of 51.70 kDa.
Activity
Immobilized Biotinylated Human GPRC5D Nanodisc, His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate(5ug/ml). Dose response curve for Anti-GPRC5D Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA (QC Test).
Product Tag
C-His
Expression System
HEK293
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22 um filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
Stability And Storage
Valid for 6 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Biotinylated Human GPRC5D Nanodisc, His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate(5ug/ml). Dose response curve for Anti-GPRC5D Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA (QC Test). Immobilized Biotinylated Human GPRC5D Nanodisc, His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate(5ug/ml). Dose response curve for Anti-GPRC5D Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA (QC Test).
Loaded Biotinylated Human GPRC5D Nanodisc, His Tag on Streptavidin-Biosensor can bind Anti-GPRC5D Antibody, hFc Tag with an affinity constant of 2.21 nM as determined in BLI assay (Gator® Prime). Loaded Biotinylated Human GPRC5D Nanodisc, His Tag on Streptavidin-Biosensor can bind Anti-GPRC5D Antibody, hFc Tag with an affinity constant of 2.21 nM as determined in BLI assay (Gator® Prime).
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