Biotinylated Human GPRC5D Protein-VLP (GPR-HM05PB)
Catalog: GPR-HM05PB
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Product Description | Recombinant Biotinylated Human GPRC5D Protein-VLP is expressed from HEK293.It contains Met1-Val345. |
Product Background |
Chimeric antigen receptor (CAR) T cells, a type of cell-based immunotherapy, have shown some promising results in multiple myeloma, a bone marrow cancer.The orphan G protein–coupled receptor, class C group 5 member D (GPRC5D), normally expressed only in the hair follicle, Using quantitative immunofluorescence, we determined that GPRC5D protein is expressed on CD138 MM cells from primary marrow samples with a distribution that was similar to, but independent of, BCMA. |
Product Category |
Recombinant Protein |
Target |
GPRC5D |
Synonyms | GPRC5D; MGC129713; MGC129714; orphan G-protein coupled receptor |
Accession | |
Species |
Human |
Amino Acid Range |
Met1-Val345 |
Molecular Weight | The target protein has a predicted MW of 39.6 kDa. |
Activity |
Immobilized Human CD3E&CD3D, hFc Tag at 1ug/ml (100ul/well) on the plate. Add serial dilutions of Anti-CD3×GPRC5D Bispecific Antibody, hFc Tag in 10% Human Serum and then add various dilution ratio of Biotinylated Human GPRC5D VLP. Detection was performed using HRP-conjugated streptavidin with the EC50 of 75.5ng/ml determined by ELISA. The affinity constant of 0.30 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Express System |
HEK293 |
Purity | > 95% as determined by HPLC |
Endotoxin | Less than 1EU per ul by the LAL method. |
Form | Liquid |
Shipping | Shipped with dry ice. |
Formulation | Supplied as 0.22um filtered solution in PBS, 300mM L-Arginine (pH 7.4). |
Stability And Storage | Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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