Sensorgram showing increasing binding responses over time for different analyte concentrations, followed by gradual dissociation after injection ends.
  • Express System: HEK293
  • Purity: > 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human GPRC5D VLP at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-GPRC5D Antibody, hFc Tag with the EC50 of 7.4ng/ml determined by ELISA (QC Test). The affinity constant of 0.28 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Met1-Val345
Catalog #GPR-HM05PB
Regular price $1,765.00
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Biotinylated Human GPRC5D VLP (GPR-HM05PB) Summary

Product Description

Recombinant Biotinylated Human GPRC5D VLP is expressed from HEK293.It contains Met1-Val345 (It may have cross reaction with anti-His antibody).

Product Background

G protein–coupled receptor, family C, group 5, member D (GPRC5D) is an orphan receptor that is primarily expressed in plasma cells and hard keratinized tissues, with low expression in normal human tissues. GPRC5D expression is enriched in malignant plasma cells and is associated with markers of high-risk myeloma.

Product Category

Recombinant Protein / Antibody discovery / Multiple transmembrane proteins / VLP

Biotinylated Human GPRC5D VLP (GPR-HM05PB) Specifications/Details

Protein
GPRC5D
Synonyms
GPRC5D; MGC129713; MGC129714; orphan G-protein coupled receptor
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Met1-Val345
Molecular Weight
The target protein has a predicted MW of 39.6 kDa.
Activity
Immobilized Biotinylated Human GPRC5D VLP at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-GPRC5D Antibody, hFc Tag with the EC50 of 7.4ng/ml determined by ELISA (QC Test). The affinity constant of 0.28 nM as determined in SPR assay (Biacore T200).
Expression System
HEK293
Purity
> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22um filtered solution in PBS, 300mM L-arginine (pH 7.4).
Stability And Storage
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Biotinylated Human GPRC5D VLP captured on CM5 Chip via Streptavidin can bind Anti-GPRC5D Antibody with an affinity constant of 0.28 nM as determined in SPR assay (Biacore T200). Biotinylated Human GPRC5D VLP captured on CM5 Chip via Streptavidin can bind Anti-GPRC5D Antibody with an affinity constant of 0.28 nM as determined in SPR assay (Biacore T200).
Immobilized Biotinylated Human GPRC5D VLP at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-GPRC5D Antibody, hFc Tag with the EC50 of 7.4ng/ml determined by ELISA (QC Test). Immobilized Biotinylated Human GPRC5D VLP at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-GPRC5D Antibody, hFc Tag with the EC50 of 7.4ng/ml determined by ELISA (QC Test).
The purity of Biotinylated Human GPRC5D VLP is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human GPRC5D VLP is greater than 95% as determined by SEC-HPLC.
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