• Express System: E.coli
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Gly25-Thr305(HLA-A*01:01), Ile21-Met119(B2M) and EVDPIGHLY peptide
Catalog #MHC-HE461B
Price
Regular price $341.00
Regular price Sale price $341.00
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Human HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Monomer Protein (MHC-HE461B) Summary

Product Description

 Recombinant Biotinylated Human HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Monomer Protein is expressed from E.coli with His tag and Avi Tag at the C-terminus.It contains Gly25-Thr305(HLA-A*01:01), Ile21-Met119(B2M) and EVDPIGHLY peptide.

Product Background

 Melanoma antigen gene A3 (MAGE-A3) is one of the most immunogenic cancer testis antigens and is common in various types of cancers. MAGE-A3 can be considered as a predictor for poor prognosis and an option for vaccine immunotherapy in patients with PCa.

Product Category

 Recombinant Protein / Antibody discovery / MHC / Peptide-MHCs

HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Specifications

Protein
 HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY)
Synonyms
 Melanoma-associated antigen 3; MZ2-D; MZ2D; MZ2-D; CT1.3; MAGE-3

MHC Form

 Monomer

Allele

 HLA-A*01:01
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Gly25-Thr305(HLA-A*01:01), Ile21-Met119(B2M) and EVDPIGHLY peptide
Molecular Weight
 The protein has a predicted MW of 35.84 kDa (HLA-A*01:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
Product Tag
 C-His-Avi

Fluorophore

 Biotin-labeled
Expression System
 E.coli
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in 20mM Tris, 200mM NaCl (pH 8.0).
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Biotinylated Human HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Monomer is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Monomer is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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