Name: Biotinylated Human HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Monomer Protein (MHC-HM427B)
Brand: KactusBio
SKU: MHC-HM427B-100UG
Price: 310.0 USD
Availability: InStock

Biotinylated Human HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Monomer Protein (MHC-HM427B)

Immobilized Biotinylated Human HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Monomer, His Tag at 1ug/ml(100ul/well) on the streptavidin precoated plate(5ug/ml). Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 10.9ng/ml determined by ELISA (QC Test).

Biotinylated Human HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Monomer is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
Activity: Immobilized Biotinylated Human HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Monomer, His Tag at 1ug/ml(100ul/well) on the streptavidin precoated plate(5ug/ml). Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 10.9ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Gly25-Thr305(HLA-A*01:01), Ile21-Met119(B2M) and EVDPIGHLY peptide

Catalog #MHC-HM427B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human HLA-A01:01&B2M&MAGE-A3 (EVDPIGHLY) Monomer Protein is expressed from HEK293 with His tag and Avi Tag at the C-terminus.It contains Gly25-Thr305(HLA-A01:01), Ile21-Met119(B2M) and EVDPIGHLY peptide.
Product Background	Melanoma antigen gene A3 (MAGE-A3) is one of the most immunogenic cancer testis antigens and is common in various types of cancers. MAGE-A3 can be considered as a predictor for poor prognosis and an option for vaccine immunotherapy in patients with PCa.
Product Category	Recombinant Protein / Antibody discovery / MHC

Protein	HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Monomer
Synonyms	Melanoma-associated antigen 3; MZ2-D; MZ2D; MZ2-D; CT1.3; MAGE-3
MHC Form	Monomer
Allele	HLA-A*01:01
Accession	Q5SUL5(HLA-A*01:01)&P61769(B2M)&EVDPIGHLY
Species	Human
Biotinylated	yes
Amino Acid Range	Gly25-Thr305(HLA-A*01:01), Ile21-Met119(B2M) and EVDPIGHLY peptide
Molecular Weight	The protein has a predicted MW of 50.30 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Monomer, His Tag at 1ug/ml(100ul/well) on the streptavidin precoated plate(5ug/ml). Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 10.9ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.； -80°C for 3 months after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Human HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Monomer, His Tag at 1ug/ml(100ul/well) on the streptavidin precoated plate(5ug/ml). Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 10.9ng/ml determined by ELISA (QC Test).
	Biotinylated Human HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Monomer is greater than 95% as determined by SEC-HPLC.