- Express System: HEK293
- Product Tag: C-His-Avi
- Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
- Activity: Immobilized Biotinylated Human HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Monomer, His Tag at 1ug/ml(100ul/well) on the streptavidin precoated plate(5ug/ml). Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 10.9ng/ml determined by ELISA (QC Test). See testing image for detail.
- Amino Acid Range: Gly25-Thr305(HLA-A*01:01), Ile21-Met119(B2M) and EVDPIGHLY peptide
Product Description |
Recombinant Biotinylated Human HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Monomer Protein is expressed from HEK293 with His tag and Avi Tag at the C-terminus.It contains Gly25-Thr305(HLA-A*01:01), Ile21-Met119(B2M) and EVDPIGHLY peptide. |
Product Background |
Melanoma antigen gene A3 (MAGE-A3) is one of the most immunogenic cancer testis antigens and is common in various types of cancers. MAGE-A3 can be considered as a predictor for poor prognosis and an option for vaccine immunotherapy in patients with PCa. |
Product Category |
Recombinant Protein |
Target |
HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Monomer |
Synonyms |
Melanoma-associated antigen 3; MZ2-D; MZ2D; MZ2-D; CT1.3; MAGE-3 |
MHC Form |
Monomer |
Allele |
HLA-A*01:01 |
Accession |
Q5SUL5(HLA-A*01:01)&P61769(B2M)&EVDPIGHLY |
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Gly25-Thr305(HLA-A*01:01), Ile21-Met119(B2M) and EVDPIGHLY peptide |
Molecular Weight |
The protein has a predicted MW of 50.30 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Monomer, His Tag at 1ug/ml(100ul/well) on the streptavidin precoated plate(5ug/ml). Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 10.9ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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