• Express System: E.coli
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and FMNKFIYEI peptide
Catalog #MHC-HE017B
Price
Regular price $34100
Regular price Sale price $34100
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Size
Avantor VWR Fisher Scientific

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Biotinylated Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer Protein (MHC-HE017B) Summary

Product Description

 Recombinant Biotinylated Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and FMNKFIYEI peptide.

Product Background

 Alpha-fetoprotein (AFP), a specific liver cancer marker, T cells expressing AFP-CAR selectively degranulated, released cytokines, and lysed liver cancer cells that were HLA-A*02:01 /AFP while sparing cells from multiple tissue types that were negative for either expressed proteins.CAR T-cell immunotherapy targeting intracellular/secreted solid tumor antigens can elicit a potent antitumor response.

HLA-A*02:01&B2M&AFP (FMNKFIYEI) Specifications

Protein
 HLA-A*02:01&B2M&AFP (FMNKFIYEI)
Synonyms
 Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-feto; AFP; HPAFP; AFPD; FETA;
MHC Form
 Monomer
Allele
 HLA-A*02:01
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and FMNKFIYEI peptide
Molecular Weight
 The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
Product Tag
 C-His-Avi
Fluorophore
 Biotin-labeled
Expression System
 E.coli
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in 20mM Tris, 200mM NaCl (pH 8.0).
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Biotinylated Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...