Biotinylated Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Monomer Protein (MHC-HM458B)
Biotinylated Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Monomer Protein (MHC-HM458B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and NLVPMVATV peptide
Catalog #MHC-HM458B
Regular price $341.00
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Biotinylated Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Monomer Protein (MHC-HM458B) Summary

Product Description

Recombinant Biotinylated Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and NLVPMVATV peptide.

Product Background

Human cytomegalovirus (CMV) is one of the major causes of severe complications in immunocompromised patients, such as recipients of hematopoietic cells, solid organ transplantation, and HIV-infected individuals. CMV is also a major pathogen in congenital infection. It can cause life-threatening diseases and severe neurological sequelae in newborn infants. CMV phosphoprotein pp65 is thought to be a major antigen for CMV-specific cellular immunity.

Product Category

Recombinant Protein / Antibody discovery / MHC / Peptide-MHCs

HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Specifications

Protein
HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV)
Synonyms
MHC; HLA-A*02:01; CMV pp65

MHC Form

Monomer

Allele

HLA-A*02:01
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and NLVPMVATV peptide
Molecular Weight
The protein has a predicted MW of 50.35 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Bis-Tris PAGE result.

Product Tag

C-His-Avi
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biotinylated Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Monomer is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Monomer is greater than 95% as determined by SEC-HPLC.
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