Biotinylated Human HLA-A*02:01&B2M&CMVpp65 (NLVPMVATV) Monomer Protein (MHC-HE013B)
Catalog: MHC-HE013B
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| Product Description | Recombinant Biotinylated Human HLA-A*02:01&B2M&CMVpp65 (NLVPMVATV) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus.It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and NLVPMVATV peptide. |
Product Background |
Human cytomegalovirus (CMV), a β-herpes virus with a double-stranded DNA, infects a wide variety of cells and establishes latency in the host. CMVpp65, a tegument protein of the herpes virus CMV, is the main viral antigen found in peripheral blood mononuclear cells (PBMCs) after viral infection and may activate cell-mediated immunity, accounting for 70-90% of the cytotoxic CD8+ T cells' (CTLs) response to CMV. Among the pp65-derived CTL epitope peptides, the 9-mer peptide 495NLVPMVATV503 (CMVpp65 495-503 peptide) is the most immunogenic T cell epitope predominantly displayed on HLA-A*02:01, the most common MHC-I allele in the population. |
Product Category |
Recombinant Protein |
Target |
HLA-A*02:01&B2M&CMVpp65 (NLVPMVATV) Monomer |
| Accession | |
| MHC Form | Monomer |
| Allele | HLA-A*02:01 |
Species |
Human |
Amino Acid Range |
Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and NLVPMVATV peptide |
| Molecular Weight | The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Tris-Bis PAGE result. |
Product Tag |
C-His-Avi |
Express System |
E.coli |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22 um filtered solution in 20mM Tris, 200mM NaCl (pH 8.0). Normally 8% mannitol is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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The purity of Biotinylated Human HLA-A*02:01&B2M&CMVpp65 (NLVPMVATV) Monomer is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Human HLA-A*02:01&B2M&CMVpp65 (NLVPMVATV) Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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