Name: Biotinylated Human HLA-A*02:01&B2M&CMVpp65 (NLVPMVATV) Monomer Protein (MHC-HE013B)
Brand: KactusBio
SKU: MHC-HE013B-100UG
Price: 310.0 USD
Availability: InStock

Biotinylated Human HLA-A*02:01&B2M&CMVpp65 (NLVPMVATV) Monomer Protein (MHC-HE013B)

Immobilized Biotinylated HLA-A*02:01&B2M&CMVpp65 (NLVPMVATV) Monomer, His Tag at 1ug/ml(100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 12.1ng/ml determined by ELISA.

Biotinylated Human HLA-A*02:01&B2M&CMVpp65 (NLVPMVATV) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human HLA-A*02:01&B2M&CMVpp65 (NLVPMVATV) Monomer is greater than 95% as determined by SEC-HPLC.

Express System: E.coli
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated HLA-A*02:01&B2M&CMVpp65 (NLVPMVATV) Monomer, His Tag at 1ug/ml(100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 12.1ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and NLVPMVATV peptide

Catalog #MHC-HE013B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human HLA-A02:01&B2M&CMVpp65 (NLVPMVATV) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus.It contains Gly25-Thr305 (HLA-A02:01), Ile21-Met119 (B2M) and NLVPMVATV peptide.
Product Background	Human cytomegalovirus (CMV), a β-herpes virus with a double-stranded DNA, infects a wide variety of cells and establishes latency in the host. CMVpp65, a tegument protein of the herpes virus CMV, is the main viral antigen found in peripheral blood mononuclear cells (PBMCs) after viral infection and may activate cell-mediated immunity, accounting for 70-90% of the cytotoxic CD8+ T cells' (CTLs) response to CMV. Among the pp65-derived CTL epitope peptides, the 9-mer peptide 495NLVPMVATV503 (CMVpp65 495-503 peptide) is the most immunogenic T cell epitope predominantly displayed on HLA-A*02:01, the most common MHC-I allele in the population.
Product Category	Recombinant Protein / Antibody discovery / MHC

Protein	HLA-A*02:01&B2M&CMVpp65 (NLVPMVATV) Monomer
Synonyms	CMVpp65; NLVPMVATV
MHC Form	Monomer
Allele	HLA-A*02:01
Accession	A0A140T913(HLA-A*02:01)&P61769(B2M)&NLVPMVATV
Species	Human
Biotinylated	yes
Amino Acid Range	Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and NLVPMVATV peptide
Molecular Weight	The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
Activity	Immobilized Biotinylated HLA-A*02:01&B2M&CMVpp65 (NLVPMVATV) Monomer, His Tag at 1ug/ml(100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 12.1ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Expression System	E.coli
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in 20mM Tris, 200mM NaCl (pH 8.0). Normally 8% mannitol is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated HLA-A*02:01&B2M&CMVpp65 (NLVPMVATV) Monomer, His Tag at 1ug/ml(100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 12.1ng/ml determined by ELISA.
	Biotinylated Human HLA-A*02:01&B2M&CMVpp65 (NLVPMVATV) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human HLA-A*02:01&B2M&CMVpp65 (NLVPMVATV) Monomer is greater than 95% as determined by SEC-HPLC.