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Biotinylated Human HLA-A*02:01&B2M&IGKC (FIFPPSDEQL) Monomer Protein (MHC-HE030B) Summary
Product Description |
Recombinant Biotinylated Human HLA-A*02:01&B2M&IGKC (FIFPPSDEQL) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus.It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and FIFPPSDEQL peptide. |
Product Background |
IGKC (Immunoglobulin kappa constant) is a segment of the kappa light chain. This region is important for the stability and function of the antibody molecule, as it helps in the proper folding and assembly of the immunoglobulin. Additionally, the kappa constant region may also be involved in the interaction between the antibody and other immune system components. |
Product Category |
Recombinant Protein / Antibody discovery / MHC / Peptide-MHCs |
HLA-A*02:01&B2M&IGKC (FIFPPSDEQL) Specifications
Protein |
HLA-A*02:01&B2M&IGKC (FIFPPSDEQL) |
Synonyms |
FIFPPSDEQL |
MHC Form |
Monomer |
Allele |
HLA-A*02:01 |
Accession |
A0A140T913(HLA-A*02:01)&P61769(B2M)&FIFPPSDEQL |
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and FIFPPSDEQL peptide |
Molecular Weight |
The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human HLA-A*02:01&B2M&IGKC (FIFPPSDEQL) Monomer, His Tag at 1ug/ml (100 ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 27.4ng/ml determined by ELISA. |
Product Tag |
C-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Formulation |
Supplied as 0.22 um filtered solution in 20 mM Tris, 200 mM NaCl (pH 8.0). |
Stability And Storage |
Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Biotinylated Human HLA-A*02:01&B2M&IGKC (FIFPPSDEQL) Monomer, His Tag at 1ug/ml (100 ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 27.4ng/ml determined by ELISA. |
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Biotinylated Human HLA-A*02:01&B2M&IGKC (FIFPPSDEQL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human HLA-A*02:01&B2M&IGKC (FIFPPSDEQL) Monomer is greater than 95% as determined by SEC-HPLC. |
Additional Information
Other Available Proteins:
Finding available proteins...
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